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SKU:MA1034
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:DCS-3
Application:ICC, WB
Price: $200.00
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Overview

Product Name Anti-Cyclin D2 Antibody (Monoclonal, DCS-3)
SKU/Catalog Number MA1034
Description Mouse IgG monoclonal antibody for Cyclin D2, cyclin D2 (CCND2) detection. Tested with WB, ICC in Human;mouse. No cross reactivity with other proteins.
Cite This Product Anti-Cyclin D2 Antibody (Monoclonal, DCS-3) DCS-3 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1034)
Replacement Item This antibody may replace the following items: sc-166288|sc-181|sc-181-G|sc-376676|sc-452|sc-53637|sc-56305|sc-65551|sc-717|sc-718|sc-754|sc-755 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG2a
Validated Species Human, Mouse
Application ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Recombinant human cyclin D2 protein.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number DCS-3
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG2a

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Ccnd2
Protein Name G1/S-specific cyclin-D2
Molecular Weight 32826 MW
Protein Function Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity). .
Sequence Similarities Belongs to the cyclin family. Cyclin D subfamily.
Subcellular Localization Nucleus . Cytoplasm . Membrane . Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members. .
Uniprot ID Q04827
Alternative Names G1/S-specific cyclin-D2;Vin-1 proto-oncogene;Ccnd2;Vin-1;
Research Areas |cell biology|cell cycle|cyclins| cell biology|cyclin d family| epigenetics and nuclear signaling| cancer|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for G1/S-specific cyclin-D2

Inaba et al.(1992) used murine cDNA clones for 3 cyclin D genes that are normally expressed during the G1 phase of the cell cycle to clone the cognate human genes. D-type cyclins(cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. cyclin D2(CCND2) gene ia assigned to 12p13.
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Publications

Sun L, Carr Al, Li P, Lee J, Mcgregor M, Li L. Biochem Biophys Res Commun. 2014 Jul 11;449(4):444-8. Doi: 10.1016/J.Bbrc.2014.05.048. Epub 2014 May 20. Characterization Of The Human Oncogene Scl/Tal1 Interrupting Locus (Stil) Mediated Sonic Hedgeh...

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.