Product Info Summary
SKU: | A01744-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Cyclin D3 CCND3 Antibody
SKU/Catalog Number
A01744-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Cyclin D3 CCND3 Antibody catalog # A01744-2. Tested in WB,ICC/IF,Flow Cytometry applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin D3 CCND3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01744-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human MYPT1 around the phosphorylation site of T853.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01744-2 is reactive to CCND3 in Human, Mouse
Applications
A01744-2 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
32.52kDa
Background of Cyclin D3
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Chen Y., Mol. Cell. Biol. 17:6049-6056(1997).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Zheng L., Mol. Cell. Biol. 19:5417-5428(1999).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000
ICC: 1:50-1:200
FC: 1:10-1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of CCND3 using Anti-Cyclin D3 CCND3 Antibody (A01744-2).
ICC staining Cyclin D3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Cyclin D3 CCND3 Antibody A01744-2
Western blot analysis of Cyclin D3 on different lysates using anti-Cyclin D3 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For CCND3 (Source: Uniprot.org, NCBI)
Gene Name
CCND3
Full Name
G1/S-specific cyclin-D3
Weight
32.52kDa
Superfamily
cyclin family
Alternative Names
CCND3; Cyclin D3; D3-type cyclin; G1/S-specific cyclin D3; G1/S-specific cyclin-D3 CCND3 cyclin D3 G1/S-specific cyclin-D3|D3-type cyclin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCND3, check out the CCND3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCND3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin D3 CCND3 Antibody (A01744-2)
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No publications found for A01744-2
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