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SKU:PA1909
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-CYP2E1 Antibody
SKU/Catalog Number PA1909
Description Rabbit IgG polyclonal antibody for Cytochrome P450 2E1(CYP2E1) detection. Tested with WB, IHC-P in Mouse;Rat.
Cite This Product Anti-CYP2E1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1909)
Replacement Item This antibody may replace the following items: sc-133491|sc-26830|sc-26834 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of mouse CYP2E1 (94-112aa KNEFSGRGDIPVFQEYKNK), identical to the related rat sequence.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Cyp2e1
Protein Name Cytochrome P450 2E1
Molecular Weight 56805 MW
Protein Function Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. .
Tissue Specificity Highest level in the liver and to a lesser extent in the kidney, with a higher level in the male kidney than in the female.
Subcellular Localization Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Uniprot ID Q05421
Alternative Names Cytochrome P450 2E1;1.14.13.-;4-nitrophenol 2-hydroxylase;1.14.13.n7;CYPIIE1;Cytochrome P450-ALC;Cytochrome P450-J;Cyp2e1;Cyp2e, Cyp2e-1;
Research Areas |cardiovascular|lipids / lipoproteins|lipid metabolism|cytochromes| signal transduction|drug metabolism|alcohol metabolism| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|lipases|mitochondrial metabolism|types of disease|cancer|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Cytochrome P450 2E1

CYP2E1, also known as P450IIE1, is a member of the P450IIE subfamily which is ethanol-inducible. It has at least 1 gene which is mapped to 10q24.3-qter, and a second is likely in rat and in man. Both the rat and human proteins encoded by this gene contain 493 amino acids and calculated molecular masses of 56,634 and 56,916 daltons, respectively. In addition, genetic polymorphisms in the 5-prime flanking region of the human P450IIE1 gene affected its binding of transacting factor and changed its transcriptional regulation, which may lead to interindividual differences of microsomal drug oxidation activity. P450IIE1 is an important enzyme for the catalysis of the conversion of ethanol to acetaldehyde and to acetate in humans,and it is also involved in the metabolism of nitrosamines. Due to the possible correlation of P450IIE1 genes with malignancy, clinical studies of RFLP patterns of these genes in cancer may be useful.

Anti-CYP2E1 Antibody Images

Click the images to enlarge.

Anti-CYP2E1 Antibody
Anti-CYP2E1 antibody, PA1909, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Mouse Liver Tissue Lysate
Anti-CYP2E1 Antibody
Anti-CYP2E1 antibody, PA1909, IHC(P)
IHC(P): Rat Liver Tissue
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Publications

Chen X, Sun Ck, Han Gz, Peng Jy, Li Y, Liu Yx, Lv Yy, Liu Kx, Zhou Q, Sun Hj. World J Gastroenterol. 2009 Apr 21;15(15):1829-35. Protective Effect Of Tea Polyphenols Against Paracetamol-Induced Hepatotoxicity In Mice Is Significantly Correlated Wi...
Fan X, Jiang Y, Wang Y, Tan H, Zeng H, Wang Y, Chen P, Qu A, Gonzalez Fj, Huang M, Bi H. Drug Metab Dispos. 2014 Dec;42(12):1982-90. Doi: 10.1124/Dmd.114.059535. Epub 2014 Sep 12. Wuzhi Tablet (Schisandra Sphenanthera Extract) Protects Against Ace...
Jiang Y, Fan X, Wang Y, Chen P, Zeng H, Tan H, Gonzalez Fj, Huang M, Bi H. Toxicol Sci. 2015 Jan;143(1):107-15. Doi: 10.1093/Toxsci/Kfu216. Epub 2014 Oct 14. Schisandrol B Protects Against Acetaminophen-Induced Hepatotoxicity By Inhibition Of Cyp-...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cyp2e1 antibody, cytochrome p450 2e1 antibody