|Application:||IHC-P, ICC, WB|
Data & Images
|Product Name||Anti-CYP2U1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Cytochrome P450 2U1(CYP2U1) detection. Tested with WB, IHC-P, ICC in Human.|
|Cite This Product||Anti-CYP2U1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2022)|
|Replacement Item||This antibody may replace the following items: sc-48991|sc-99215|sc-393368|sc-393071 from Santa Cruz Biotechnology.|
|Application||IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human CYP2U1(457-473aa EKPEDFYPNRFLDDQGQ).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cytochrome P450 2U1|
|Molecular Weight||61987 MW|
|Protein Function||Catalyzes the hydroxylation of arachidonic acid, docosahexaenoic acid and other long chain fatty acids. May modulate the arachidonic acid signaling pathway and play a role in other fatty acid signaling processes. .|
|Tissue Specificity||Widely expressed with stronger expression in thymus, heart and cerebellum. .|
|Subcellular Localization||Endoplasmic reticulum membrane ; Multi-pass membrane protein . Microsome membrane .|
|Alternative Names||Cytochrome P450 2U1;220.127.116.11;CYP2U1;|
|Research Areas|||cardiovascular|lipids / lipoproteins|lipid metabolism|cytochromes| signal transduction| cardiovascular|fatty acids| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|lipases|mitochondrial metabolism|types of disease|cancer|redox metabolism|fatty acid oxidation||
Background for Cytochrome P450 2U1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CYP2U1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CYP2U1 Antibody Images
Click the images to enlarge.
Lane 1: HELA Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: MM453 Cell Lysate
Lane 4: COLO320 Cell Lysate
Lane 5: HT1080 Cell Lysate
IHC(P): Human Intestinal Cancer Tissue
ICC: HELA Cell
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,