Product Info Summary
SKU: | PA1556 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Cystathionase/CTH Antibody
View all Cystathionase Antibodies
SKU/Catalog Number
PA1556
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cystathionase/CTH Antibody catalog # PA1556. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cystathionase/CTH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1556)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cystathionase, different from the related mouse and rat sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1556 is reactive to CTH in Human, Mouse, Rat
Applications
PA1556 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
45 kDa
Calculated molecular weight
44.508kDa
Background of Cystathionase
Cystathionine gamma-lyase (or cystathionase) is an enzyme which breaks down cystathionine into cysteine and alpha-ketobutyrate. The International Radiation Hybrid Mapping Consortium mapped the CTH gene to chromosome 1. The CTH gene had earlier been assigned to chromosome 16 by study of somatic cell hybrids. It is demonstrated that hydrogen sulfide (H2S) is physiologically generated by CTH.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CTH using anti-CTH antibody (PA1556).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human HCCT tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTH antigen affinity purified polyclonal antibody (Catalog # PA1556) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTH at approximately 45 kDa. The expected band size for CTH is at 45 kDa.
Click image to see more details
Figure 2. IF analysis of CTH using anti-CTH antibody (PA1556).
CTH was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CTH Antibody (PA1556) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of JK cells using anti-CTH antibody (PA1556).
Overlay histogram showing JK cells stained with PA1556 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTH Antibody (PA1556, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CTH (Source: Uniprot.org, NCBI)
Gene Name
CTH
Full Name
Cystathionine gamma-lyase
Weight
44.508kDa
Superfamily
trans-sulfuration enzymes family
Alternative Names
cystathionase (cystathionine gamma-lyase); cystathionine gamma-lyase; cysteine desulfhydrase; EC 4.4.1; EC 4.4.1.1; gamma-cystathionase; homoserine deaminase; homoserine dehydratase; MGC9471 CTH cystathionine gamma-lyase cystathionine gamma-lyase|cystathionase (cystathionine gamma-lyase)|cysteine desulfhydrase|cysteine-protein sulfhydrase|gamma-cystathionase|homoserine deaminase|homoserine dehydratase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTH, check out the CTH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cystathionase/CTH Antibody (PA1556)
Hello CJ!
PA1556 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
High C-X-C motif chemokine 5 expression is associated with malignant phenotypes of prostate cancer cells via autocrine and paracrine pathways
Heme Oxygenase-1 Promotes Delayed Wound Healing in Diabetic Rats
Endogenous carbon monoxide downregulates hepatic cystathionine-?-lyase in rats with liver cirrhosis
Caffeic Acid Phenethyl Ester inhibit Hepatic Fibrosis by Nitric Oxide Synthase and Cystathionine Gamma-Lyase in Rats
Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway
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Customer Q&As
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1 Customer Q&As for Anti-Cystathionase/CTH Antibody
Question
Could you please let us know whether the antibodies marked as suitable for paraffin-embedded tissue works also on vibratome sections?
Verified customer
Asked: 2021-02-02
Answer
Unfortunately, the Anti-Cystathionase/CTH Antibody (PA1556) hasn't been used on vibratome sections. Please run a pilot test to see if it can work.
Boster Scientific Support
Answered: 2021-02-03