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SKU:PA1369
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, ICC, WB
Price: $200.00
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Overview

Product Name Anti-Cystatin C Antibody
SKU/Catalog Number PA1369
Description Rabbit IgG polyclonal antibody for Cystatin-C(CST3) detection. Tested with WB, IHC-P, ICC in Human.
Cite This Product Anti-Cystatin C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1369)
Replacement Item This antibody may replace the following items: sc-25564|sc-133138|sc-27269|sc-5655 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application IHC-P, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Cystatin C(45-63aa EEEGVRRALDFAVGEYNKA).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CST3
Protein Name Cystatin-C
Molecular Weight 15799 MW
Protein Function As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
Tissue Specificity Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. .
Subcellular Localization Secreted .
Uniprot ID P01034
Alternative Names Cystatin-C;Cystatin-3;Gamma-trace;Neuroendocrine basic polypeptide;Post-gamma-globulin;CST3;
Research Areas |tags & cell markers|cell type markers|tumor associated| cell biology|apoptosis|intracellular|caspases etc|inhibitors| stem cells|neural stem cells|extracellular| cardiovascular|atherosclerosis|thrombosis|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Cystatin-C

Cystatin C or cystatin 3, a protein encoded by the CST3 gene, is mainly used as a biomarker of kidney function. Recently, it has been studied for its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer's disease. By human-rodent somatic cell hybridizations, Abrahamson et al.(1989) mapped the human CST3 to chromosome 20. Cystatin C was originally described as a constituent of normal cerebrospinal fluid(CSF) and of urine from patients with renal failure(Grubb and Lofberg, 1982). It is present in a number of neuroendocrine cells and its concentration in the CSF was reported to be 5.5 times that in plasma of healthy adults(Lofberg and Grubb, 1979; Lofberg et al., 1981; Lofberg et al., 1983). Grubb and Lofberg(1982) detected the protein in human pituitary gland, and suggested that it is part of the gastroenteropancreatic neuroendocrine system.

Anti-Cystatin C Antibody Images

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Anti-Cystatin C Antibody
Anti-Cystatin C antibody, PA1369, Western blotting
Lane 1: Recombinant Human Cystatin C Protein 10ng
Lane 2: Recombinant Human Cystatin C Protein 5ng
Lane 3: Recombinant Human Cystatin C Protein 2.5ng
Anti-Cystatin C Antibody
Anti-Cystatin C antibody, PA1369, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-Cystatin C Antibody
Anti-Cystatin C antibody, PA1369, ICC
ICC: MCF-7 Cell
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is cystatin-c antibody