Anti-Cytokeratin 16 KRT16 Rabbit Monoclonal Antibody

Cytokeratin 16 antibody

Boster Bio Anti-Cytokeratin 16 KRT16 Rabbit Monoclonal Antibody catalog # M03393. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M03393
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-Cytokeratin 16 KRT16 Rabbit Monoclonal Antibody

View all Cytokeratin 16 Antibodies

SKU/Catalog Number

M03393

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Cytokeratin 16 KRT16 Rabbit Monoclonal Antibody catalog # M03393. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cytokeratin 16 KRT16 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03393)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

EDC-11

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Cytokeratin 16

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M03393 is reactive to KRT16 in Human

Applications

M03393 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

51.268kDa

Background of Cytokeratin 16

Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For KRT16 (Source: Uniprot.org, NCBI)

Gene Name

KRT16

Full Name

Keratin, type I cytoskeletal 16

Weight

51.268kDa

Superfamily

intermediate filament family

Alternative Names

CK16; CK-16; cytokeratin 16; cytokeratin-16; FNEPPK; focal non-epidermolytic palmoplantar keratoderma; K16; K1CP; keratin 16; keratin, type I cytoskeletal 16; keratin-16; KRT16A; NEPPK KRT16 CK16, FNEPPK, K16, K1CPA, NEPPK, PC1, KRT16 keratin 16 keratin, type I cytoskeletal 16|cytokeratin 16|focal non-epidermolytic palmoplantar keratoderma|keratin 16, type I

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on KRT16, check out the KRT16 Infographic

KRT16 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KRT16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Cytokeratin 16 KRT16 Rabbit Monoclonal Antibody

Question

Will M03393 anti-Cytokeratin 16 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

As indicated on the product datasheet, M03393 anti-Cytokeratin 16 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-18

Question

Will anti-Cytokeratin 16 Rabbit Monoclonal antibody M03393 work for IF with liver?

F. Yang

Verified customer

Asked: 2020-02-27

Answer

According to the expression profile of liver, KRT16 is highly expressed in liver. So, it is likely that anti-Cytokeratin 16 Rabbit Monoclonal antibody M03393 will work for IF with liver.

Boster Scientific Support

Answered: 2020-02-27

Question

Do you have a BSA free version of anti-Cytokeratin 16 Rabbit Monoclonal antibody M03393 available?

L. Zhao

Verified customer

Asked: 2018-05-25

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Cytokeratin 16 Rabbit Monoclonal antibody M03393 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-05-25

Question

Is a blocking peptide available for product anti-Cytokeratin 16 Rabbit Monoclonal antibody (M03393)?

Verified Customer

Verified customer

Asked: 2018-04-20

Answer

We do provide the blocking peptide for product anti-Cytokeratin 16 Rabbit Monoclonal antibody (M03393). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-04-20

Question

We are currently using anti-Cytokeratin 16 Rabbit Monoclonal antibody M03393 for human tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2017-07-06

Answer

The anti-Cytokeratin 16 Rabbit Monoclonal antibody (M03393) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-07-06

Question

My question regarding product M03393, anti-Cytokeratin 16 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

R. Mangal

Verified customer

Asked: 2014-09-09

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03393 anti-Cytokeratin 16 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-09-09

Order DetailsPrice
M03393

100μL

$370
M03393-30ul

30μL sample

$99
M03393-carrier-free

Carrier Free

$370

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Free Secondary Antibody

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M03393
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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