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SKU:MA1026
Clonality:Monoclonal
Clone Number:CY-90
Application:IHC-P, IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-Cytokeratin Peptide 18 Antibody (Monoclonal, CY-90)
Description Mouse IgG monoclonal antibody for Cytokeratin Peptide 18, keratin 18 (KRT18) detection. Tested with WB, IHC-P, IHC-F in Human; rat. No cross reactivity with other proteins.
Cite This Product Anti-Cytokeratin Peptide 18 Antibody (Monoclonal, CY-90) CY-90 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1026)
Replacement Item This antibody may replace the following items: sc-101727|sc-17031|sc-17031-R|sc-17032|sc-17032-R|sc-28264|sc-31700|sc-31701|sc-32329|sc-32722|sc-398871|sc-51582|sc-51583|sc-52325|sc-53254|sc-53256|sc-53262|sc-53265|sc-56376|sc-58727|sc-58825|sc-6259|sc-70917|sc-70925|sc-70926|sc-70928|sc-70939 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Rat
Application IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number CY-90
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Krt18
Protein Name Keratin, type I cytoskeletal 18
Molecular Weight 47761 MW
Protein Function When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection (By similarity). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. .
Tissue Specificity Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue. .
Sequence Similarities Belongs to the intermediate filament family.
Subcellular Localization Nucleus matrix . Nucleus, nucleolus . Cytoplasm .
Uniprot ID Q5BJY9
Alternative Names Keratin, type I cytoskeletal 18;Cytokeratin-18;CK-18;Keratin-18;K18;Krt18 ;Krt1-18 ;
Research Areas |signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|cytokeratin| signal transduction|structures|cytoskeleton|intermediate filaments|class i|microtubules|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Keratin, type I cytoskeletal 18

Intermediate filaments(IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18(KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis.

Anti-Cytokeratin Peptide 18 Antibody (Monoclonal, CY-90) Images

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Anti-Cytokeratin Peptide 18 Antibody (Monoclonal, CY-90)
Anti-Cytokeratin Peptide 18 antibody (monoclonal), MA1026, Western blotting
WB: MCF-7 Cell Lysate
Anti-Cytokeratin Peptide 18 Antibody (Monoclonal, CY-90)
Anti-Cytokeratin Peptide 18 antibody (monoclonal), MA1026, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-Cytokeratin Peptide 18 Antibody (Monoclonal, CY-90)
Anti-Cytokeratin Peptide 18 antibody (monoclonal), MA1026, IHC(P)
IHC(P): Human Rectal Cancer Tissue
Anti-Cytokeratin Peptide 18 Antibody (Monoclonal, CY-90)
Anti-Cytokeratin Peptide 18 antibody (monoclonal), MA1026, IHC(P)
IHC(P): Human Mammary Cancer Tissue

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Publications

Zhang, Y., Zhang, L., Yu, C.F., Du, X.Y., Liu, X.R., Liu, J.Z.,…, & Cao, B.Y. (2017). Effects of interferon tau on endometrial epithelial cells in caprine in vitro. Gene Expression Patterns. Advance online publication. doi: 10.1016/j.gep.2017.06.010

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to ck18 antibody, cytokeratin 18 antibody, cytokeratin-18 antibody, keratin 18 antibody, keratin-18 antibody