|Application:||IHC-P, IHC-F, WB|
Data & Images
|Product Name||Anti-Cytokeratin Peptide 18 Antibody (Monoclonal, CY-90)|
|Description||Mouse IgG monoclonal antibody for Cytokeratin Peptide 18, keratin 18 (KRT18) detection. Tested with WB, IHC-P, IHC-F in Human; rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-Cytokeratin Peptide 18 Antibody (Monoclonal, CY-90) CY-90 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1026)|
|Replacement Item||This antibody may replace the following items: sc-101727|sc-17031|sc-17031-R|sc-17032|sc-17032-R|sc-28264|sc-31700|sc-31701|sc-32329|sc-32722|sc-398871|sc-51582|sc-51583|sc-52325|sc-53254|sc-53256|sc-53262|sc-53265|sc-56376|sc-58727|sc-58825|sc-6259|sc-70917|sc-70925|sc-70926|sc-70928|sc-70939 from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
|Application||IHC-P, IHC-F, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Keratin, type I cytoskeletal 18|
|Molecular Weight||47761 MW|
|Protein Function||When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection (By similarity). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. .|
|Tissue Specificity||Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue. .|
|Sequence Similarities||Belongs to the intermediate filament family.|
|Subcellular Localization||Nucleus matrix . Nucleus, nucleolus . Cytoplasm .|
|Alternative Names||Keratin, type I cytoskeletal 18;Cytokeratin-18;CK-18;Keratin-18;K18;Krt18 ;Krt1-18 ;|
|Research Areas|||signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|cytokeratin| signal transduction|structures|cytoskeleton|intermediate filaments|class i|microtubules||
Background for Keratin, type I cytoskeletal 18
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Cytokeratin Peptide 18 Antibody (Monoclonal, CY-90) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, rat, By Heat
Immunohistochemistry(Frozen Section), 0.5-2μg/ml, Human, rat, -
Western blot, 0.5-1μg/ml, Human, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Cytokeratin Peptide 18 Antibody (Monoclonal, CY-90) Images
Click the images to enlarge.
WB: MCF-7 Cell Lysate
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Rectal Cancer Tissue
IHC(P): Human Mammary Cancer Tissue