|Sample Size:||30ug for $99, contact us for details|
|Application:||IHC-P, IHC-F, WB|
Data & Images
|Product Name||Anti-Cytokeratin Peptide 7 Antibody (Monoclonal, LDS-68)|
|Description||Mouse IgG monoclonal antibody for Cytokeratin Peptide 7, keratin 7 (KRT7) detection. Tested with WB, IHC-P, IHC-F in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-Cytokeratin Peptide 7 Antibody (Monoclonal, LDS-68) LDS-68 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1024)|
|Replacement Item||This antibody may replace the following items: sc-17116|sc-17118|sc-23876|sc-25721|sc-51585|sc-53263|sc-53264|sc-53265|sc-70936|sc-8421 from Santa Cruz Biotechnology.|
|Application||IHC-P, IHC-F, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Cytoskeletal preparation of the RT4 human bladder carcinoma cell line.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Keratin, type II cytoskeletal 7|
|Molecular Weight||50709 MW|
|Protein Function||Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. .|
|Tissue Specificity||KRT7: Expressed in cultured epidermal, bronchial andmesothelial cells but absent in colon, ectocervix and liver.Observed throughout the glandular cells in the junction betweenstomach and esophagus but is absent in the esophagus.|
|Sequence Similarities||Belongs to the intermediate filament family.|
|Subcellular Localization||KRT7: Cytoplasm|
|Alternative Names||Keratin, type II cytoskeletal 7;Cytokeratin-7;CK-7;Keratin-7;K7;Type-II keratin Kb7;Krt7 ;Kb7 , Krt2-7 ;|
Background for Keratin, type II cytoskeletal 7
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Cytokeratin Peptide 7 Antibody (Monoclonal, LDS-68) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, By Heat
Immunohistochemistry(Frozen Section), 2-4μg/ml, Human, -
Western blot, 1-2μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.