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SKU:MA1024
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:LDS-68
Application:IHC, WB
Price: $200.00
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Overview

Product Name Anti-Cytokeratin Peptide 7 Antibody (Monoclonal, LDS-68)
SKU/Catalog Number MA1024
Description Mouse IgG monoclonal antibody for Cytokeratin Peptide 7, keratin 7 (KRT7) detection. Tested with WB, IHC-P, IHC-F in Human. No cross reactivity with other proteins.
Cite This Product Anti-Cytokeratin Peptide 7 Antibody (Monoclonal, LDS-68) LDS-68 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1024)
Replacement Item This antibody may replace the following items: sc-17116|sc-17118|sc-23876|sc-25721|sc-51585|sc-53263|sc-53264|sc-53265|sc-70936|sc-8421 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human
Application IHC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Cytoskeletal preparation of the RT4 human bladder carcinoma cell line.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number LDS-68
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Krt7
Protein Name Keratin, type II cytoskeletal 7
Molecular Weight 50709 MW
Protein Function Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. .
Tissue Specificity KRT7: Expressed in cultured epidermal, bronchial andmesothelial cells but absent in colon, ectocervix and liver.Observed throughout the glandular cells in the junction betweenstomach and esophagus but is absent in the esophagus.
Sequence Similarities Belongs to the intermediate filament family.
Subcellular Localization KRT7: Cytoplasm
Uniprot ID Q6IG12
Alternative Names Keratin, type II cytoskeletal 7;Cytokeratin-7;CK-7;Keratin-7;K7;Type-II keratin Kb7;Krt7 ;Kb7 , Krt2-7 ;
Research Areas KRT7|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Keratin, type II cytoskeletal 7

KRT7 is a type II keratin of simple nonkeratinizing epithelia. The deduced 489-amino acid protein has a calculated molecular mass of about 54 kD. K7 contains 4 central alpha-helical segments with heptad repeats of hydrophobic residues characteristic of a coiled-coil region. Within this domain, K7 shares 73% homology with epidermal K6B. KRT7 gene contains 9 exons and spans more than 15.6 kb. K7 gene is mapped to chromosome 12. Keratin 7 is expressed in a wide range of epithelial structures in humans.
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to scl antibody, ck7 antibody, cytokeratin 7 antibody, cytokeratin-7 antibody, keratin 7 antibody, keratin-7 antibody