Anti-Phospho-DARPP 32 (Thr34) PPP1R1B Antibody

DARPP-32 antibody

Boster Bio Anti-Phospho-DARPP 32 (Thr34) PPP1R1B Antibody (Catalog # P03424-1). Tested in WB applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: P03424-1
Size: 100 µl
Reactive Species: Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Phospho-DARPP 32 (Thr34) PPP1R1B Antibody

View all DARPP-32 Antibodies

SKU/Catalog Number

P03424-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-DARPP 32 (Thr34) PPP1R1B Antibody (Catalog # P03424-1). Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-DARPP 32 (Thr34) PPP1R1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03424-1)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Clone Number

608

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr34 of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P03424-1 is reactive to Ppp1r1b in Mouse, Rat

Applications

P03424-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

21.781kDa

Background of DARPP-32

DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr-34. In contrast, when DARPP-32 is phosphorylated on Thr-75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

Gene/Protein Information For Ppp1r1b (Source: Uniprot.org, NCBI)

Gene Name

Ppp1r1b

Full Name

Protein phosphatase 1 regulatory subunit 1B

Weight

21.781kDa

Superfamily

protein phosphatase inhibitor 1 family

Alternative Names

DARPP32; DARPP-32; PPP1R1B; protein phosphatase 1, regulatory (inhibitor) subunit 1B; regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32) Ppp1r1b|AU040756, DARP, DARPP-32, Dar, Darpp32|protein phosphatase 1, regulatory inhibitor subunit 1B|protein phosphatase 1 regulatory subunit 1B|dopamine- and cAMP-regulated neuronal phosphoprotein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Ppp1r1b, check out the Ppp1r1b Infographic

Ppp1r1b infographic

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In this infographic, you will see the following information for Ppp1r1b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
P03424-1

100uL

$465

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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