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SKU:PA1910
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-DCC Antibody
Description Rabbit IgG polyclonal antibody for Netrin receptor DCC(DCC) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-DCC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1910)
Replacement Item This antibody may replace the following items: sc-6534|sc-6535|sc-11437 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human DCC(162-178aa EVIGEPMPTIHWQKNQQ), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name DCC
Protein Name Netrin receptor DCC
Molecular Weight 158457 MW
Protein Function Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene. .
Tissue Specificity Found in axons of the central and peripheral nervous system and in differentiated cell types of the intestine. Not expressed in colorectal tumor cells that lost their capacity to differentiate into mucus producing cells. .
Sequence Similarities Belongs to the immunoglobulin superfamily. DCC family.
Subcellular Localization Membrane; Single-pass type I membrane protein.
Uniprot ID P43146
Alternative Names Netrin receptor DCC;Colorectal cancer suppressor;Immunoglobulin superfamily DCC subclass member 1;Tumor suppressor protein DCC;DCC;IGDCC1;
Research Areas |cell biology|apoptosis|receptors|associated proteins| neuroscience|neurology process|growth and development|axonal guidance proteins|neurogenesis| epigenetics and nuclear signaling|transcription|cancer susceptibility|tumor suppressors| cancer|cell death|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Netrin receptor DCC

DCC(Deleted IN Colorectal Carcinoma) also known as CRC18 or CRCR1. The DCC gene encodes a functional receptor for netrin and mediates axon outgrowth and the steering response. Heterozygous loss-of-function mutations in DCC can result in congenital mirror movements. Alterations in DCC occur frequently in colorectal cancer. Studying a YAC contig containing the entire DCC coding region, they showed that the DCC gene spans approximately 1.4 Mb. Vogelstein(1995) stated that the precise location of the DCC gene was though to be 18q21.3. DCC is a receptor or a component of a receptor that mediates the effects of netrin-1 on commissural axons, and they complement genetic evidence for interactions between DCC and netrin homologs in C. DCC protein could be detected in varying abundance in all specimens of normal colonic mucosa analyzed as well as in all specimens of adenomatous polyps, colorectal carcinoma and colorectal liver metastases. DCC may function as a tumor-suppressor protein by inducing apoptosis in settings in which ligand is unavailable(for example, during metastasis or tumor growth beyond local blood supply) through functional caspase cascades by a mechanism that requires cleavage of DCC at asp1290. Stein et al.(2001) concluded that DCC plays a central role in netrin signaling of axon growth and guidance independent of A2B receptor activation.

Anti-DCC Antibody Images

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Anti-DCC Antibody
Anti-DCC antibody, PA1910, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Lane 3: U87 Cell Lysate
Lane 4: SW620 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: 293T Cell Lysate
Lane 7: HELA Cell Lysate
Anti-DCC Antibody
Anti-DCC antibody, PA1910, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.