Product Info Summary
SKU: | PA1963 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-DDX4/MVH Antibody
SKU/Catalog Number
PA1963
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DDX4/MVH Antibody catalog # PA1963. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDX4/MVH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1963)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human DDX4, identical to the the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1963 is reactive to Ddx4 in Human, Mouse, Rat
Applications
PA1963 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee
Observed Molecular Weight
32 kDa, 19 kDa
Calculated molecular weight
76.47kDa
Background of VASA
DDX4 (DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al. (2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog (Ddx4) resides (Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al. (2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat
Immunocytochemistry , 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-DDX4/MVH antibody, PA1963, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Mouse Testis Tissue Lysate
Lane 3: HELA Cell Lysate
Click image to see more details
Anti-DDX4/MVH antibody, PA1963, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Mouse Testis Tissue Lysate
Lane 3: Mouse Ovary Tissue Lysate
Click image to see more details
Anti-DDX4/MVH antibody, PA1963, IHC(P)
IHC(P): Rat Ovary Tissue
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Anti-DDX4/MVH antibody, PA1963, IHC(P)
IHC(P): Rat Testis Tissue
Click image to see more details
Anti-DDX4/MVH antibody, PA1963, ICC
ICC: MCF-7 Cell
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Anti-DDX4/MVH antibody, PA1963, IHC(F)
IHC(F): Rat Intestine Tissue
Click image to see more details
Figure 10. IF analysis of DDX4/MVH using anti-DDX4/MVH antibody (PA1963).
DDX4/MVH was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DDX4/MVH Antibody (PA1963) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 7. Flow Cytometry analysis of SiHa cells using anti-DDX4/MVH antibody (PA1963).
Overlay histogram showing SiHa cells stained with PA1963 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX4/MVH Antibody (PA1963, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 8. Flow Cytometry analysis of PC-3 cells using anti-DDX4/MVH antibody (PA1963).
Overlay histogram showing PC-3 cells stained with PA1963 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX4/MVH Antibody (PA1963, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 9. IHC analysis of DDX4/MVH using anti-DDX4/MVH antibody (PA1963).
DDX4/MVH was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDX4/MVH Antibody (PA1963) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Ddx4 (Source: Uniprot.org, NCBI)
Gene Name
Ddx4
Full Name
ATP-dependent RNA helicase DDX4
Weight
76.47kDa
Superfamily
DEAD box helicase family
Alternative Names
DDX4; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD box protein 4; EC 3.6.1; EC 3.6.4.13; MGC111074; probable ATP-dependent RNA helicase DDX4; Vasa homolog; VASA; VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 Ddx4|AV206478, Mv, Mvh, VASA|DEAD box helicase 4|ATP-dependent RNA helicase DDX4|D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 4|DEAD (Asp-Glu-Ala-Asp) box polypeptide 4|DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 4|DEAD box polypeptide 4|DEAD box protein 4|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4|mvh / mvasa|probable ATP-dependent RNA helicase DDX4|vasa homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ddx4, check out the Ddx4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ddx4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDX4/MVH Antibody (PA1963)
Hello CJ!
PA1963 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Evaluating differentiation potential of the human menstrual blood-derived stem cells from infertile women into oocyte-like cells
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Customer Q&As
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1 Customer Q&As for Anti-DDX4/MVH Antibody
Question
We are currently using anti-DDX4/MVH antibody PA1963 for mouse tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-10
Answer
The anti-DDX4/MVH antibody (PA1963) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-10