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SKU:PB9105
Clonality:Polyclonal
Application:IHC-P, IHC-F, WB
Price: $240.00
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Overview

Product Name Anti-Desmin Picoband™ Antibody
SKU/Catalog Number PB9105
Description Rabbit IgG polyclonal antibody for Desmin(DES) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.
Cite This Product Anti-Desmin Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9105)
Replacement Item This antibody may replace the following items: sc-130593|sc-134338|sc-14026|sc-162734|sc-23879|sc-271677|sc-34200|sc-34201|sc-365130|sc-52326|sc-58744|sc-58745|sc-65983|sc-70961|sc-7559 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human Desmin recombinant protein (Position: M1-T304). Human Desmin shares 97% amino acid (aa) sequence identity with both mouse and rat Desmin.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name DES
Protein Name Desmin
Molecular Weight 53536 MW
Protein Function Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. .
Sequence Similarities Belongs to the intermediate filament family.
Subcellular Localization Cytoplasm. Cell membrane, sarcolemma .
Uniprot ID P17661
Alternative Names Desmin;DES;
Research Areas |immunology|innate immunity|complement|classical pathway| immunology|alternative pathway|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Desmin

DES, also called desmin, is a protein that in humans is encoded by the DES gene, and this gene is mapped to 2q35. DES is the muscle-specific member of the intermediate filament (IF) protein family. It is one of the earliest myogenic markers, both in heart and somites, and is expressed in satellite stem cells and replicating myoblasts. DES is very important in muscle cell architecture and structure since it connects many components of the cytoplasm. It may be also play an important role in mitochondria function. What’s more, DES provides attachments between the terminal Z disc and membrane-associated proteins to form a force-transmitting system that parallels the thin filaments at myotendinous junctions.

Anti-Desmin Picoband™ Antibody Images

Click the images to enlarge.

Anti-Desmin Picoband™ Antibody
Anti-Desmin Picoband antibody, PB9105-1.jpg
All lanes: Anti Desmin (PB9105) at 0.5ug/ml
WB: Recombinant Human Desmin Protein 0.5ng
Predicted bind size: 36KD
Observed bind size: 36KD
Anti-Desmin Picoband™ Antibody
Anti-Desmin Picoband antibody, PB9105-2.jpg
All lanes: Anti Desmin (PB9105) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Rat Cardiac MuscleTissue Lysate at 50ug
Lane 3: Mouse Skeletal Muscle Tissue Lysate at 50ug
Lane 4: Mouse Cardiac MuscleTissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: HT1080 Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: HEPA Whole Cell Lysate at 40ug
Lane 9: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 53KD
Anti-Desmin Picoband™ Antibody
Anti-Desmin Picoband antibody, PB9105-3.JPG
IHC(F): Rat Cardiac Muscle Tissue
Anti-Desmin Picoband™ Antibody
Anti-Desmin Picoband antibody, PB9105-4.JPG
IHC(F): Mouse Cardiac Muscle Tissue
Anti-Desmin Picoband™ Antibody
Anti-Desmin Picoband antibody, PB9105-5.JPG
IHC(P): Human Intestinal Cancer Tissue
Anti-Desmin Picoband™ Antibody
Anti-Desmin Picoband antibody, PB9105-6.JPG
IHC(P): Mouse Skeletal Muscle Tissue
Anti-Desmin Picoband™ Antibody
Anti-Desmin Picoband antibody, PB9105-7.JPG
IHC(P): Rat Cardiac Muscle Tissue
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Publications

Wang W, Fan M, Zhang L, Liu Sh, Sun L, Wang Cy. Biomed Mater. 2009 Apr;4(2):025011. Doi: 10.1088/1748-6041/4/2/025011. Epub 2009 Mar 4. Compatibility Of Hyaluronic Acid Hydrogel And Skeletal Muscle Myoblasts.
Zhang F, Deng B, Wen J, Chen K, Liu W, Ye S, Huang H, Jiang S, Xiong Y. Biochem Biophys Res Commun. 2015 Mar 6;458(2):375-80. Doi: 10.1016/J.Bbrc.2015.01.120. Epub 2015 Jan 31. Ppar?? And Myod Are Differentially Regulated By Myostatin In Adipose-De...
Liu Hh, Li L, Chen X, Cao W, Zhang Rp, Yu Hy, Xu F, He H, Wang Jw. Cytotechnology. 2011 Aug;63(4):399-406. Doi: 10.1007/S10616-011-9356-7. Epub 2011 May 26. Characterization Of In Vitro Cultured Myoblasts Isolated From Duck (Anas Platyrhynchos) Em...
Chen S, Fang Z, Zhu Z, Deng A, Liu J, Zhang C. J Huazhong Univ Sci Technolog Med Sci. 2009 Dec;29(6):715-9. Doi: 10.1007/S11596-009-0608-0. Epub 2009 Dec 29. Protective Effect Of Sulodexide On Podocyte Injury In Adriamycin Nephropathy Rats.
Liu J, Liu Y, Xu Y, Wang M, Zhu Q, Li W, Wang X, Liu J, Ma B, Wu K. Exp Mol Pathol. 2013 Feb;94(1):79-83. Doi: 10.1016/J.Yexmp.2012.10.012. Epub 2012 Oct 23. Neurotransmitter Noradrenaline Downregulate Cytoskeletal Protein Expression Of Vsmcs.
Sondag Gr, Salihoglu S, Lababidi Sl, Crowder Dc, Moussa Fm, Abdelmagid Sm, Safadi Ff. J Cell Physiol. 2014 Jul;229(7):955-66. Doi: 10.1002/Jcp.24512. Osteoactivin Induces Transdifferentiation Of C2C12 Myoblasts Into Osteoblasts.
Zhang Sc, Zheng Yh, Yu Pp, Min Th, Yu Fx, Ye C, Xie Yk, Zhang Qy. World J Gastroenterol. 2012 Jul 28;18(28):3696-704. Doi: 10.3748/Wjg.V18.I28.3696. Lentiviral Vector-Mediated Down-Regulation Of Il-17A Receptor In Hepatic Stellate Cells Results In...
Liu Hh, Wang Jw, Yu Hy, Zhang Rp, Chen X, Jin Hb, Dai F, Li L, Xu F. Appl Microbiol Biotechnol. 2012 Jun;94(5):1255-63. Doi: 10.1007/S00253-012-3908-4. Epub 2012 Feb 18. Injection Of Duck Recombinant Follistatin Fusion Protein Into Duck Muscle Tis...
Wang Xq, Yang Wj, Yang Z, Shu G, Wang Sb, Jiang Qy, Yuan L, Wu Ts. Plos One. 2012;7(3):E32537. Doi: 10.1371/Journal.Pone.0032537. Epub 2012 Mar 13. The Differential Proliferative Ability Of Satellite Cells In Lantang And Landrace Pigs.
Yan J, Gan L, Yang H, Sun C. Mol Biol Rep. 2013 Apr;40(4):3197-202. Doi: 10.1007/S11033-012-2395-0. Epub 2012 Dec 29. The Proliferation And Differentiation Characteristics Of Co-Cultured Porcine Preadipocytes And Muscle Satellite Cells In Vitro.
Gao Cq, Zhi R,, Yang Z,, Li Hc, Yan Hc, Wang Xq. J Cell Biochem. 2015 Nov;116(11):2637-48. Doi: 10.1002/Jcb.25212. Low Dose Of Igf-I Increases Cell Size Of Skeletal Muscle Satellite Cells Via Akt/S6K Signaling Pathway.
Xu X, Qian H, Zhu W, Zhang X, Yan Y, Wang M, Xu W. J Mol Med (Berl). 2010 Nov;88(11):1181-90. Doi: 10.1007/S00109-010-0659-5. Epub 2010 Aug 10. Isolation Of Cancer Stem Cells From Transformed Human Mesenchymal Stem Cell Line F6.
Pan X, Chen Z, Huang R, Yao Y, Ma G. Plos One. 2013;8(4):E60335. Doi: 10.1371/Journal.Pone.0060335. Epub 2013 Apr 1. Transforming Growth Factor ??1 Induces The Expression Of Collagen Type I By Dna Methylation In Cardiac Fibroblasts.
Qian Q, Qian H, Zhang X, Zhu W, Yan Y, Ye S, Peng X, Li W, Xu Z, Sun L, Xu W. Stem Cells Dev. 2012 Jan;21(1):67-75. Doi: 10.1089/Scd.2010.0519. Epub 2011 Jun 1. 5-Azacytidine Induces Cardiac Differentiation Of Human Umbilical Cord-Derived Mesenchy...
Jiang R, Xu W, Zhu W, Chen M, Qian H, Qiao C, Yang H, Wang X, Chen Y. Oncol Rep. 2006 Nov;16(5):1021-8. Histological Type Of Oncogenity And Expression Of Cell Cycle Genes In Tumor Cells From Human Mesenchymal Stem Cells.
Longxi P, Buwu F, Yuan W, Sinan G. Plos One. 2011;6(10):E26500. Doi: 10.1371/Journal.Pone.0026500. Epub 2011 Oct 28. Expression Of P53 In The Effects Of Artesunate On Induction Of Apoptosis And Inhibition Of Proliferation In Rat Primary Hepatic St...
Wu R, Gu B, Zhao X, Tan Z, Chen L, Zhu J, Zhang M. Hum Cell. 2013 Mar;26(1):19-27. Doi: 10.1007/S13577-011-0022-3. Epub 2011 Jun 15. Derivation Of Multipotent Nestin(+)/Cd271 (-)/Stro-1 (-) Mesenchymal-Like Precursors From Human Embryonic Stem Cel...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.