Product Info Summary
SKU: | A05374-3 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-DFF40 Dffb Antibody
SKU/Catalog Number
A05374-3
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DFF40 Dffb Antibody (Catalog # A05374-3). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
DFF40 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-DFF40 Dffb Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05374-3)
Host
Rabbit
Contents
DFF40 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
SK7
Isotype
IgG
Immunogen
DFF40 antibody was raised against a peptide corresponding to 18 amino acids near the center of murine CAD. The immunogen is located within amino acids 130 - 180 of DFF40.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Multiple isoforms of GLS2 are known to exist.
Reactive Species
A05374-3 is reactive to DFFB in Human, Mouse, Rat
Applications
A05374-3 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
39.11kDa
Background of DFF40/CAD
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DFF40 antibody can be used for detection of DFF40 by Western blot 0.5 μg/mL. A 40 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DFF40 in HeLa (H)
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Immunocytochemistry of DFF40 in Jurkat cells with DFF40 antibody at 5 μg/mL.
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Immunofluorescence of DFF40 in K562 cells with DFF40 antibody at 20 μg/ml.
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Immunofluorescence of DFF40 in K562 cells with DFF40 antibody at 20 μg/mL.
Green: DFF40 Antibody (A05374-3)
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For DFFB (Source: Uniprot.org, NCBI)
Gene Name
DFFB
Full Name
DNA fragmentation factor subunit beta
Weight
39.11kDa
Alternative Names
CAD; CADCaspase-activated nuclease; Caspase-activated deoxyribonuclease; Caspase-activated DNase; CPAN; CPANDNA fragmentation factor 40 kDa subunit; DFF2; DFF40; DFF-40DFF40DFF2DNA fragmentation factor subunit beta; DFFB; DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase); EC 3.- DFFB CAD, CPAN, DFF-40, DFF2, DFF40 DNA fragmentation factor subunit beta DNA fragmentation factor subunit beta|DNA fragmentation factor 40 kDa subunit|DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)|caspase-activated DNase|caspase-activated deoxyribonuclease|caspase-activated nuclease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DFFB, check out the DFFB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DFFB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DFF40 Dffb Antibody (A05374-3)
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