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SKU:PA2023
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-DISC1 Antibody
Description Rabbit IgG polyclonal antibody for Disrupted in schizophrenia 1 protein(DISC1) detection. Tested with WB in Human.
Cite This Product Anti-DISC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2023)
Replacement Item This antibody may replace the following items: sc-47988 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2016!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human DISC1(74-91aa EESHHSESRARQCGLDSR).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name DISC1
Protein Name Disrupted in schizophrenia 1 protein
Gene Full Name Disrupted in schizophrenia 1 protein
Protein Function Involved in the regulation of multiple aspects ofembryonic and adult neurogenesis. Required for neural progenitorproliferation in the ventrical/subventrical zone during embryonicbrain development and in the adult dentate gyrus of thehippocampus. Participates in the Wnt-mediated neural progenitorproliferation as a positive regulator by modulating GSK3B activityand CTNNB1 abundance. Plays a role as a modulator of the AKT-mTORsignaling pathway controlling the tempo of the process of newbornneurons integration during adult neurogenesis, including neuronpositioning, dendritic development and synapse formation. Inhibitsthe activation of AKT-mTOR signaling upon interaction withCCDC88A. Regulates the migration of early-born granule cellprecursors toward the dentate gyrus during the hippocampaldevelopment. Plays a role, together with PCNT, in the microtubulenetwork formation..
Tissue Specificity Ubiquitous. Highly expressed in the dentategyrus of the hippocampus. Also expressed in the temporal andparahippocampal cortices and cells of the white matter..
Subcellular Localization Cytoplasm. Cytoplasm, cytoskeleton.Cytoplasm, cytoskeleton, microtubule organizing center,centrosome. Cell junction, synapse, postsynaptic cell membrane,postsynaptic density. Colocalizes with NDEL1 inthe perinuclear region and the centrosome (By similarity).Localizes to punctate cytoplasmic foci which overlap in part withmitochondria. Colocalizes with PCNT at the centrosome..
Uniprot ID DISC1: Q9NRI5
Alternative Names Disrupted in schizophrenia 1 protein; DISC1; KIAA0457;
Research Areas neuroscience|neurology process|neurodegenerative disease|alzheimer's disease|neurogenesis|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Disrupted in schizophrenia 1 protein

DISC1(Disrupted in Schizophrenia 1), is a protein that is encoded by the DISC1 gene in humans. Ma et al.(2002) determined that the mouse Disc1 gene maps to chromosome 8 in a region with homology of synteny to human chromosome 1q42. Ozeki et al.(2003) demonstrated that rodent Disc1 expression displayed pronounced developmental regulation, with the highest levels in late embryonic life during development of the cerebral cortex. Millar et al.(2005) showed that DISC1 interacts with the UCR2 domain of phosphodiesterase-4B, implicated in susceptibility to schizophrenia, and that elevation of cellular cAMP leads to dissociation of PDE4B from DISC1 and in increase in PDE4B activity.

Anti-DISC1 Antibody Images

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Anti-DISC1 Antibody
Anti-DISC1 antibody, PA2023, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: SHG Cell Lysate

Customer Reviews

Review by Shubhankar Nath
Quality

Source: Biocompare.com

"The Best DISC1 Antibody on the Market"

--Shubhankar Nath, MOLECULAR BIOSCIENCES, UNIVERSITY OF TEXAS, GRADUATE STUDENT

Applications Western Blot
Sample Jurkat cell lysate
Detection Chemiluminescence

"We tested several anti-DISC1 antibodies that are commercially available on the market. However, we found that this rabbit polyclonal anti-DISC1 antibody from Bosterbio company is the best one. We have used this antibody for IF, WB and IP purposes in NIH3T3 and Jurkat cell lines...The antibody detected a single band of protein at around 100 KD. Cheap, polyclonal, multipurpose use, fast shipping...The best DISC1 antibody in the market that can be used for IP, IF and WB."

(Posted on 1/18/2016)
Write a review for PA2023

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.