Data & Images
|Product Name||Anti-DISC1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Disrupted in schizophrenia 1 protein(DISC1) detection. Tested with WB in Human.|
|Cite This Product||Anti-DISC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2023)|
|Replacement Item||This antibody may replace the following items: sc-47988 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2016!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human DISC1(74-91aa EESHHSESRARQCGLDSR).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Disrupted in schizophrenia 1 protein|
|Gene Full Name||Disrupted in schizophrenia 1 protein|
|Protein Function||Involved in the regulation of multiple aspects ofembryonic and adult neurogenesis. Required for neural progenitorproliferation in the ventrical/subventrical zone during embryonicbrain development and in the adult dentate gyrus of thehippocampus. Participates in the Wnt-mediated neural progenitorproliferation as a positive regulator by modulating GSK3B activityand CTNNB1 abundance. Plays a role as a modulator of the AKT-mTORsignaling pathway controlling the tempo of the process of newbornneurons integration during adult neurogenesis, including neuronpositioning, dendritic development and synapse formation. Inhibitsthe activation of AKT-mTOR signaling upon interaction withCCDC88A. Regulates the migration of early-born granule cellprecursors toward the dentate gyrus during the hippocampaldevelopment. Plays a role, together with PCNT, in the microtubulenetwork formation..|
|Tissue Specificity||Ubiquitous. Highly expressed in the dentategyrus of the hippocampus. Also expressed in the temporal andparahippocampal cortices and cells of the white matter..|
|Subcellular Localization||Cytoplasm. Cytoplasm, cytoskeleton.Cytoplasm, cytoskeleton, microtubule organizing center,centrosome. Cell junction, synapse, postsynaptic cell membrane,postsynaptic density. Colocalizes with NDEL1 inthe perinuclear region and the centrosome (By similarity).Localizes to punctate cytoplasmic foci which overlap in part withmitochondria. Colocalizes with PCNT at the centrosome..|
|Uniprot ID||DISC1: Q9NRI5
|Alternative Names||Disrupted in schizophrenia 1 protein; DISC1; KIAA0457;|
|Research Areas||neuroscience|neurology process|neurodegenerative disease|alzheimer's disease|neurogenesis||
Background for Disrupted in schizophrenia 1 protein
At Boster we strive to provide the best Anti-DISC1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Application||Concentration*||Tested Species||Predicted Species||Antigen Retrieval|
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-DISC1 Antibody Images
Click the images to enlarge.
Lane 1: U87 Cell Lysate
Lane 2: SHG Cell Lysate
- Review by Shubhankar Nath
"The Best DISC1 Antibody on the Market"
--Shubhankar Nath, MOLECULAR BIOSCIENCES, UNIVERSITY OF TEXAS, GRADUATE STUDENT
Applications Western Blot Sample Jurkat cell lysate Detection Chemiluminescence
"We tested several anti-DISC1 antibodies that are commercially available on the market. However, we found that this rabbit polyclonal anti-DISC1 antibody from Bosterbio company is the best one. We have used this antibody for IF, WB and IP purposes in NIH3T3 and Jurkat cell lines...The antibody detected a single band of protein at around 100 KD. Cheap, polyclonal, multipurpose use, fast shipping...The best DISC1 antibody in the market that can be used for IP, IF and WB."
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,