Anti-Dickkopf-related protein 1 DKK1 Antibody

Dkk-1 antibody

Boster Bio Anti-Dickkopf-related protein 1 DKK1 Antibody catalog # PA1462. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1462
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Dickkopf-related protein 1 DKK1 Antibody

View all Dkk-1 Antibodies

SKU/Catalog Number

PA1462

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Dickkopf-related protein 1 DKK1 Antibody catalog # PA1462. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Dickkopf-related protein 1 DKK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1462)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human DKK1, different from the related mouse and rat sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1462 is reactive to Dkk1 in Human, Mouse, Rat

Applications

PA1462 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

29.298kDa

Background of Dkk-1

DKK1, Dickkopf-related protein 1, is a protein that in humans is encoded by the DKK1 gene. This gene encodes a protein that is a member of the dickkopf family. By genomic sequence analysis, determined that the DKK1 gene contains 4 exons. The DKK1 gene is mapped to 10q11.2 using FISH. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For Dkk1 (Source: Uniprot.org, NCBI)

Gene Name

Dkk1

Full Name

Dickkopf-related protein 1

Weight

29.298kDa

Superfamily

dickkopf family

Alternative Names

dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like Dkk1|mdkk-1|dickkopf WNT signaling pathway inhibitor 1|dickkopf-related protein 1|dickkopf homolog 1|dickkopf-1|dickkopf-like protein 1|dkk-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Dkk1, check out the Dkk1 Infographic

Dkk1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Dkk1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Dickkopf-related protein 1 DKK1 Antibody

Question

Can you provide information about the crystal structures of PA1462?

Verified customer

Asked: 2021-01-15

Answer

Unfortunately, we do not have crystal structures available for the Anti-Dickkopf-Related Protein 1 DKK1 Antibody (PA1462).

Boster Scientific Support

Answered: 2021-01-19

Question

you antibody using your anti-DKK1 antibody for positive regulation of cell death studies. Has this antibody been tested with western blotting on a431 whole cell lysates? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-03-03

Answer

I appreciate your inquiry. This PA1462 anti-DKK1 antibody is tested on human placenta tissue, a431 whole cell lysates, hela whole cell lysates, a549 whole cell lysates, rat spleen tissue, mouse thymus tissue. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-03-03

Question

We have seen staining in human fetal kidney. Any tips? Is anti-DKK1 antibody supposed to stain fetal kidney positively?

Verified Customer

Verified customer

Asked: 2019-06-06

Answer

Based on literature fetal kidney does express DKK1. Based on Uniprot.org, DKK1 is expressed in decidua, leiomyosarcoma, fetal kidney, cerebellum, brain, among other tissues. Regarding which tissues have DKK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Fetal kidney, Pubmed ID: 10570958
Leiomyosarcoma, Pubmed ID: 10383463

Boster Scientific Support

Answered: 2019-06-06

Question

My team were satisfied with the WB result of your anti-DKK1 antibody. However we have observed positive staining in fetal kidney secreted. using this antibody. Is that expected? Could you tell me where is DKK1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-03-07

Answer

From literature, fetal kidney does express DKK1. Generally DKK1 expresses in secreted. Regarding which tissues have DKK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Fetal kidney, Pubmed ID: 10570958
Leiomyosarcoma, Pubmed ID: 10383463

Boster Scientific Support

Answered: 2019-03-07

Question

We are currently using anti-DKK1 antibody PA1462 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-01-30

Answer

The anti-DKK1 antibody (PA1462) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-01-30

Order DetailsPrice
PA1462

100μg

$370
PA1462-10ug

10μg sample (liquid)

$99
PA1462-Biotin

100 μg Biotin conjugated

$570
PA1462-Cy3

100 μg Cy3 conjugated

$570
PA1462-Dylight488

100 μg Dylight488 conjugated

$570
PA1462-Dylight550

100 μg Dylight550 conjugated

$570
PA1462-Dylight594

100 μg Dylight594 conjugated

$570
PA1462-FITC

100 μg FITC conjugated

$570
PA1462-HRP

100 μg HRP conjugated

$570
PA1462-APC

100 μg APC conjugated

$670
PA1462-PE

100 μg PE conjugated

$670
PA1462-carrier-free

Carrier Free

$370

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