Anti-DNA PKcs pT 2609 Antibody
|Product Name||Anti-DNA PKcs pT 2609 Antibody|
|Description||Anti-DNA PKcs pT 2609 Antibody tested for IHC, WB in Human.|
|Cite This Product||Anti-DNA PKcs pT 2609 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00645)|
|Contents/Buffer||Each vial contains 50% glycerol and 0.09% sodium azide.|
|Immunogen||Synthetic peptide corresponding to a portion of human DNA-PK.|
Assay Dilutions Overview
Western Blot, 1:500 - 1:2, 000, human, chimpanzee
Images And Assay Conditions
DNA-PK was detected in paraffin-embedded sections of human prostate tissues using rabbit anti- DNA-PK Antigen Affinity purified polyclonal antibody (Catalog # A00645) at 10 Î¼g/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
DNA-PK was detected in paraffin-embedded sections of human testis tissues using rabbit anti- DNA-PK Antigen Affinity purified polyclonal antibody (Catalog # A00645) at 10 Î¼g/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Western blot analysis of DNA-PK expression in MW marker (lane 1), HELA whole cell lysates (lane 2), mouse spleen extract (lane 3), rat spleen extract (lane 4) and CHO-K1 whole cell lysates (lane 5). DNA-PK at 350KD was detected using rabbit anti- DNA-PK Antigen Affinity purified polyclonal antibody (Catalog # A00645) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info (Source: Uniprot.org)
|Protein Name||DNA-dependent protein kinase catalytic subunit|
|Alternative Names||DNA-dependent protein kinase catalytic subunit;DNA-PK catalytic subunit;DNA-PKcs;188.8.131.52;DNPK1;p460;PRKDC;HYRC, HYRC1;|
|Subcellular Localization||Nucleus. Nucleus, nucleolus.|
|Molecular Weight||469089 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation. .|
*You can search these to find other products in these research areas.
|Background||PRKDC(Protein Kinase DNA-Activated Catalytic Subunit), also called DNAPK, HYRC1, p350 or DNPK1, is an enzyme that in humans is encoded by the PRKDC gene. DNA-PKcs belongs to the phosphatidylinositol 3-kinase-related kinase protein family. Satoh et al.(1997) mapped the MCM4 gene to 8q11.2 by FISH. Based on the close proximity of the PRKDC and MCM4 genes, it was assumed that the PRKDC gene also maps to this location. Anderson and Lees-Miller(1992) noted that DNA-PK had been shown in vitro to phosphorylate several transcription factors, suggesting that it functions in cell homeostasis by modulating transcription. Daniel et al.(1999) demonstrated that the PRKDC protein participates in retroviral DNA integration, which is catalyzed by the viral protein integrase.|
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