Anti-DNA-PKcs Rabbit Monoclonal Antibody


SKU M00645
Size 100ug/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number EIA-16
Host Rabbit
Applications IF, WB

Overview

Product Name Anti-DNA-PKcs Rabbit Monoclonal Antibody
SKU/Catalog Number M00645
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug/vial
Description Anti-DNA-PKcs Rabbit Monoclonal Antibodytested for IF, IHC, ICC, WB in Human, Mouse, Rat
Cite This Product Anti-DNA-PKcs Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00645)
Host Rabbit
Contents/Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Immunogen A synthesized peptide derived from human DNA-PKcs
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Images And Assay Conditions

Western blot analysis of DNA-PKcs expression in HeLa cell lysate (M00645).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKDC monoclonal antibody (Catalog # M00645) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKDC

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P78527
Gene Name PRKDC
Protein Name DNA-dependent protein kinase catalytic subunit
Alternative Names DNA-dependent protein kinase catalytic subunit;DNA-PK catalytic subunit;DNA-PKcs;2.7.11.1;DNPK1;p460;PRKDC;HYRC, HYRC1;
Subcellular Localization Nucleus. Nucleus, nucleolus.
Molecular Weight 469089 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation. .
Research Areas Human, Mouse, Rat

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Monoclonal antibody for DNA PKCS/PRKDC detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat DNA PKCS/PRKDC information: Molecular Weight: 469089 MW; Subcellular Localization: Nucleus. Nucleus, nucleolus.
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In stock
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M00645
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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Publications

Sun JS, Yang XH. Exp Ther Med. 2018 Apr;15(4):3295-3301. doi: 10.3892/etm.2018.5826. Epub 2018 Feb 1. Expression of DNA-dependent protein kinase catalytic subunit in laryngeal squamous cell carcinoma and its importance
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