Anti-DNA-PKcs Rabbit Monoclonal Antibody
|Reactivity||Human, Mouse, Rat|
|Product Name||Anti-DNA-PKcs Rabbit Monoclonal Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.|
|Description||Anti-DNA-PKcs Rabbit Monoclonal Antibodytested for IF, IHC, ICC, WB in Human, Mouse, Rat|
|Cite This Product||Anti-DNA-PKcs Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00645)|
|Contents/Buffer||Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|Immunogen||A synthesized peptide derived from human DNA-PKcs|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Images And Assay Conditions
Western blot analysis of DNA-PKcs expression in HeLa cell lysate (M00645).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKDC monoclonal antibody (Catalog # M00645) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKDC
Protein Target Info (Source: Uniprot.org)
|Protein Name||DNA-dependent protein kinase catalytic subunit|
|Alternative Names||DNA-dependent protein kinase catalytic subunit;DNA-PK catalytic subunit;DNA-PKcs;220.127.116.11;DNPK1;p460;PRKDC;HYRC, HYRC1;|
|Subcellular Localization||Nucleus. Nucleus, nucleolus.|
|Molecular Weight||469089 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation. .|
|Research Areas||Human, Mouse, Rat
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