Anti-DPYD Antibody

DPYD antibody

Boster Bio Anti-DPYD Antibody catalog # PA1761. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1761
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-DPYD Antibody

View all DPYD Antibodies

SKU/Catalog Number

PA1761

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DPYD Antibody catalog # PA1761. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DPYD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1761)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human DPYD, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1761 is reactive to DPYD in Human, Mouse, Rat

Applications

PA1761 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

11 kDa

Calculated molecular weight

111.401kDa

Background of DPYD

DPYD (Dihydropyrimidine Dehydrogenase), also called DPD, is an enzyme that in humans is encoded by the DPYD gene. The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. The structure of the DPYD gene contains 23 exons spanning about 950 kb. Using somatic cell hybrid strategies, the DPYD gene is mapped to the centromeric region of chromosome 1 between 1p22 and 1q21. By fluorescence in situ hybridization, the DPYD gene is mapped to 1p22. The highest level of DPD was found in monocytes followed by that in lymphocytes, granulocytes, and platelets, whereas no significant activity of DPD could be detected in erythrocytes. The activity of DPD in peripheral blood mononuclear cells was intermediate between that observed in monocytes and lymphocytes. By cDNA microarray, Western blot analysis, and luciferase reporter assay, the transcription factor LSF was identified as a positive regulator of DPYD.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For DPYD (Source: Uniprot.org, NCBI)

Gene Name

DPYD

Full Name

Dihydropyrimidine dehydrogenase [NADP(+)]

Weight

111.401kDa

Superfamily

dihydropyrimidine dehydrogenase family

Alternative Names

DHP; DHPDHASE; dihydropyrimidine dehydrogenase [NADP+]; dihydropyrimidine dehydrogenase; Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase; DPDMGC132008; EC 1.3.1.2; MGC70799 DPYD DHP, DHPDHASE, DPD dihydropyrimidine dehydrogenase dihydropyrimidine dehydrogenase [NADP(+)]|dihydrothymine dehydrogenase|dihydrouracil dehydrogenase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DPYD, check out the DPYD Infographic

DPYD infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DPYD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-DPYD Antibody

Question

Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-DPYD antibody PA1761. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-27

Question

Can you help my question with product PA1761, anti-DPYD antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-01-09

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1761 anti-DPYD antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-01-09

Question

Will anti-DPYD antibody PA1761 work on primate WB with cervix carcinoma?

H. Dhar

Verified customer

Asked: 2018-06-27

Answer

Our lab technicians have not validated anti-DPYD antibody PA1761 on primate. You can run a BLAST between primate and the immunogen sequence of anti-DPYD antibody PA1761 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate cervix carcinoma in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-06-27

Question

Would PA1761 anti-DPYD antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

M. Huang

Verified customer

Asked: 2017-11-28

Answer

You can see on the product datasheet, PA1761 anti-DPYD antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-11-28

Question

We are currently using anti-DPYD antibody PA1761 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2017-09-19

Answer

The anti-DPYD antibody (PA1761) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-09-19

Question

Is this PA1761 anti-DPYD antibody reactive to the isotypes of DPYD?

P. Baker

Verified customer

Asked: 2017-04-07

Answer

The immunogen of PA1761 anti-DPYD antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human DPYD(33-52aa AKKLDKKHWKRNPDKNCFNC), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-04-07

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-DPYD antibody PA1761. Let me know if you need anything else.

B. Mangal

Verified customer

Asked: 2016-05-09

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-05-09

Order DetailsPrice
PA1761

100μg

$370
PA1761-10ug

10μg sample (liquid)

$99
PA1761-Biotin

100 μg Biotin conjugated

$570
PA1761-Cy3

100 μg Cy3 conjugated

$570
PA1761-Dylight488

100 μg Dylight488 conjugated

$570
PA1761-Dylight550

100 μg Dylight550 conjugated

$570
PA1761-Dylight594

100 μg Dylight594 conjugated

$570
PA1761-FITC

100 μg FITC conjugated

$570
PA1761-HRP

100 μg HRP conjugated

$570
PA1761-APC

100 μg APC conjugated

$670
PA1761-PE

100 μg PE conjugated

$670
PA1761-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PA1761
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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