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SKU:PA1975
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-DR4 Antibody
SKU/Catalog Number PA1975
Description Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 10A(TNFRSF10A) detection. Tested with WB in Human.
Cite This Product Anti-DR4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1975)
Replacement Item This antibody may replace the following items: sc-31344|sc-32255|sc-65312|sc-6823|sc-6824|sc-7863|sc-8411 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human DR4(110-125aa TIKLHDQSIGTQQWEH).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name TNFRSF10A
Protein Name Tumor necrosis factor receptor superfamily member 10A
Molecular Weight 50089 MW
Protein Function Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF- kappa-B. .
Tissue Specificity Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K-562 erythroleukemia cells, MCF-7 breast carcinoma cells and activated T-cells.
Sequence Similarities Contains 1 death domain.
Subcellular Localization Membrane; Single-pass type I membrane protein.
Uniprot ID O00220
Alternative Names Tumor necrosis factor receptor superfamily member 10A;Death receptor 4;TNF-related apoptosis-inducing ligand receptor 1;TRAIL receptor 1;TRAIL-R1;CD261;TNFRSF10A;APO2, DR4, TRAILR1;
Research Areas |cell biology|apoptosis|receptors|death receptors| immunology|innate immunity|cytokines|tnf superfamily|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Tumor necrosis factor receptor superfamily member 10A

TNFRSF10A(Tumor Necrosis Factor Receptor Subfamily Member 10A), also known as APO2, DR4 or TRAILR1, is a protein that in humans is encoded by the TNFRSF10A gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. By analysis of radiation hybrids, Marsters et al.(1997) mapped the DR4 gene to 8p21. The TRAIL receptor DR5, and 2 decoy receptors for TRAIL, DCR1, and DCR2, are located in the same region, suggesting that these receptors arose from recent gene duplication events. Pan et al.(1997) found that, as with FAS, TNFR1, and DR3, overexpression of DR4 induced apoptosis. However, unlike the other 3 death receptors, DR4 did not use FADD to transmit the death signal, suggesting the use of distinct proximal signaling machinery.

Anti-DR4 Antibody Images

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Anti-DR4 Antibody
Anti-DR4 antibody, PA1975, Western blotting
Lane 1: SW620 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: HT1080 Cell Lysate
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Publications

Zhang L, Niu T, Yang Sy, Lu Z, Chen B. Spine (Phila Pa 1976). 2008 Feb 15;33(4):422-7. Doi: 10.1097/Brs.0B013E318163E036. The Occurrence And Regional Distribution Of Dr4 On Herniated Disc Cells: A Potential Apoptosis Pathway In Lumbar Intervertebr...
Fu K, Ren H, Wang Y, Fei E, Wang H, Wang G. Oncogene. 2012 Mar 8;31(10):1311-22. Doi: 10.1038/Onc.2011.315. Epub 2011 Jul 25. Dj-1 Inhibits Trail-Induced Apoptosis By Blocking Pro-Caspase-8 Recruitment To Fadd.
Liu N, Zuo C, Wang X, Chen T, Yang D, Wang J, Zhu H. Oncotarget. 2014 Jul 15;5(13):4959-71. Mir-942 Decreases Trail-Induced Apoptosis Through Isg12A Downregulation And Is Regulated By Akt.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.