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SKU:PA1842
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC, WB
Price: $200.00
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Overview

Product Name Anti-DR5 Antibody
SKU/Catalog Number PA1842
Description Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 10B(TNFRSF10B) detection. Tested with WB, IHC-P in Human.
Cite This Product Anti-DR5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1842)
Replacement Item This antibody may replace the following items: sc-243110|sc-243112|sc-243107|sc-243108|sc-368705 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application IHC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human DR5(333-348aa DPTETLRQCFDDFADL).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name TNFRSF10B
Protein Name Tumor necrosis factor receptor superfamily member 10B
Molecular Weight 47878 MW
Protein Function Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF- kappa-B. Essential for ER stress-induced apoptosis. .
Tissue Specificity Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain.
Sequence Similarities Contains 1 death domain.
Subcellular Localization Membrane; Single-pass type I membrane protein.
Uniprot ID O14763
Alternative Names Tumor necrosis factor receptor superfamily member 10B;Death receptor 5;TNF-related apoptosis-inducing ligand receptor 2;TRAIL receptor 2;TRAIL-R2;CD262;TNFRSF10B;DR5, KILLER, TRAILR2, TRICK2, ZTNFR9;UNQ160/PRO186;
Research Areas |cell biology|apoptosis|receptors|death receptors| signal transduction|growth factors/hormones|tnf| cancer|growth factors|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Tumor necrosis factor receptor superfamily member 10B

TNFRSF10B(Tumor necrosis factor receptor superfamily, member 10b) is a human gene. It is also known as DR5, CD262, KILLER, TRICK2, TRICKB, ZTNFR9, TRAILR2, TRICK2A, TRICK2B, TRAIL-R2, KILLER/DR5. The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand(TNFSF10/TRAIL/APO-2L), and transduces apoptosis signal. Mice have a homologous gene, tnfrsf10b, that has been essential in the elucidation of the function of this gene in humans. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.By analysis of radiation hybrid panels, Walczak et al.(1997) mapped the TRAILR2 gene to chromosome 8p22-p21. Northern blot analysis indicated that TRAILR2 was expressed as a 4.4-kb mRNA in all tissues tested, with the highest levels of expression in peripheral blood lymphocytes, spleen, and ovary.

Anti-DR5 Antibody Images

Click the images to enlarge.

Anti-DR5 Antibody
Anti-DR5 antibody, PA1842, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: MM231 Cell Lysate
Lane 3: SGC Cell Lysate
Lane 4: HT1080 Cell Lysate
Anti-DR5 Antibody
Anti-DR5 antibody, PA1842, IHC(P)
IHC(P): Human Lung Cancer Tissue
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Publications

Liu N, Zuo C, Wang X, Chen T, Yang D, Wang J, Zhu H. Oncotarget. 2014 Jul 15;5(13):4959-71. Mir-942 Decreases Trail-Induced Apoptosis Through Isg12A Downregulation And Is Regulated By Akt.
Li J, Shen Q, Peng R, Chen R, Jiang P, Li Y, Zhang L, Lu J. J Huazhong Univ Sci Technolog Med Sci. 2010 Aug;30(4):458-63. Doi: 10.1007/S11596-010-0449-X. Epub 2010 Aug 17. Plumbagin Enhances Trail-Mediated Apoptosis Through Up-Regulation Of Death ...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to dr5 antibody, trail r2 antibody, trail-r2 antibody