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SKU:MA1037
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:MANDYS8
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-Dystrophin Antibody (Monoclonal, MANDYS8)
SKU/Catalog Number MA1037
Description Mouse IgG monoclonal antibody for Dystrophin, dystrophin (DMD) detection. Tested with WB, IHC-P in Human;mouse;rat;rabbit. No cross reactivity with other proteins.
Cite This Product Anti-Dystrophin Antibody (Monoclonal, MANDYS8) MANDYS8 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1037)
Replacement Item This antibody may replace the following items: sc-15376|sc-22027|sc-292163|sc-32792|sc-33697|sc-33698|sc-365954|sc-47760|sc-58754|sc-73592|sc-7459|sc-7461|sc-7462 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG2b
Validated Species Human, Mouse, Rabbit, Rat
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Recombinant human dystrophin fragment.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number MANDYS8
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG2b

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Dmd
Protein Name Dystrophin
Molecular Weight 425828 MW
Protein Function Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin- associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission. .
Tissue Specificity Strongly expressed in skeletal muscle and weak expression observed in newborn brain which increases in adult brain. .
Sequence Similarities Contains 2 CH (calponin-homology) domains.
Subcellular Localization Cell membrane, sarcolemma ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Cell junction, synapse, postsynaptic cell membrane . In muscle cells, sarcolemma localization requires the presence of ANK2, while localization to costameres requires the presence of ANK3. Localizes to neuromuscular junctions (NMJs). In adult muscle, NMJ localization depends upon ANK2 presence, but not in newborn animals. .
Uniprot ID P11530
Alternative Names Dystrophin;Dmd;
Research Areas |biochemicals|product range| biochemicals|chemical type|pharmacology|signaling|signal transduction|nitric oxide & oxidative stress|antioxidants|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Dystrophin

Dystrophin(DMD) gene has 79 exons spanning at least 2,300 kb(2.3 Mb). The C terminus of the dystrophin protein is encoded by a highly conserved, alternatively spliced region of the gene. beta-dystroglycan binding activity is expressed by the dystrophin fragment spanning amino acids 3026-3345 containing the ZZ domain. DMD transcript is formed by at least 60 exons; the first half of the transcript is formed by a minimum of 33 exons spanning nearly 1000 kb, and the remaining portion has at least 27 exons that may spread over a similar distance. Dystrophin gene is expressed at a higher level in primary cultures of neuronal cells than in astro-glial cells derived from adult mouse brain. overexpression of dystrophin prevents the development of the abnormal mechanical properties associated with dystrophic muscle without causing deleterious side effects.
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Will this cross react to zebrafish? Keyword: cross reaction
A: The immunogen of MA1037 shares very low homology with zebrafish, it will not cross react with zebrafish samples.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.