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SKU:PA2078
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-E-Cadherin Antibody
Description Rabbit IgG polyclonal antibody for Cadherin-1(CDH1) detection. Tested with WB in Human.
Cite This Product Anti-E-Cadherin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2078)
Replacement Item This antibody may replace the following items: sc-25250|sc-69784|sc-366443 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human E Cadherin(662-677aa DYKINLKLMDNQNKDQ).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CDH1
Protein Name Cadherin-1
Molecular Weight 97456 MW
Protein Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. .
Tissue Specificity Non-neural epithelial tissues.
Sequence Similarities Contains 5 cadherin domains.
Subcellular Localization Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma- catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
Uniprot ID P12830
Alternative Names Cadherin-1;CAM 120/80;Epithelial cadherin;E-cadherin;Uvomorulin;CD324;E-Cad/CTF1;E-Cad/CTF2;E-Cad/CTF3;CDH1;CDHE, UVO;
Research Areas |signal transduction|cytoskeleton / ecm|cell adhesion|cadherins| signal transduction|signaling pathway|calcium signaling|calcium binding proteins| cancer|invasion/microenvironment| kits/ lysates/ other|elisa kits|tumor biomarkers elisa kits|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Cadherin-1

CDH1(Cadherin 1), also known as ECAD or UVO, is a protein that in humans is encoded by the CDH1 gene. Cadherin-1 is a classical member of the cadherin superfamily. By Southern analysis of DNA from a panel of mouse-human somatic cell hybrids, Mansouri et al.(1987, 1988) assigned the UVO gene to 16q(16p11-qter). Frebourg et al.(2006) found that in human embryos CDH1 is highly expressed at 4 and 5 weeks in the frontonasal prominence and at 6 weeks in the lateral and medial nasal prominences, and is therefore expressed during critical stages of lip and palate development. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

Anti-E-Cadherin Antibody Images

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Anti-E-Cadherin Antibody
Anti-E Cadherin antibody, PA2078, Western blotting
Lane 1: A549 Cell Lysate
Lane 2: SGC Cell Lysate
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Publications

Wang J, Zhao H, Tang D, Wu J, Yao G, Zhang Q. Cancer Biother Radiopharm. 2013 May;28(4):283-8. Doi: 10.1089/Cbr.2012.1293. Epub 2013 Apr 25. Overexpressions Of Microrna-9 And Microrna-200C In Human Breast Cancers Are Associated With Lymph Node Met...
Shen R, Xie F, Shen H, Liu Q, Zheng T, Kou X, Wang D, Yang J. Plos One. 2013;8(2):E56542. Doi: 10.1371/Journal.Pone.0056542. Epub 2013 Feb 20. Negative Correlation Of Liv-1 And E-Cadherin Expression In Hepatocellular Carcinoma Cells.
Shen H, Qin H, Guo J. Mol Biol Rep. 2009 Apr;36(4):653-9. Doi: 10.1007/S11033-008-9225-4. Epub 2008 Mar 11. Concordant Correlation Of Liv-1 And E-Cadherin Expression In Human Breast Cancer Cell Mcf-7.
Jin B, Guo B, Che G, Sun Y, Liu Y, Zhang X. Microsc Res Tech. 2012 Aug;75(8):1150-3. Doi: 10.1002/Jemt.22080. Epub 2012 Jun 18. Germline Stem Cell Lineage Tracing By Free-Floating Immunofluorescent Assay Of Mouse Seminiferous Tubule.
Wang M, Zhang J, Song X, Liu W, Zhang L, Wang X, Lv C. Food Chem Toxicol. 2013 Jun;56:450-8. Doi: 10.1016/J.Fct.2013.03.004. Epub 2013 Mar 14. Astaxanthin Ameliorates Lung Fibrosis In Vivo And In Vitro By Preventing Transdifferentiation, Inhibitin...
Deng B, Zhang Y, Zhang S, Wen F, Miao Y, Guo K. Tumour Biol. 2015 Sep;36(10):8065-73. Doi: 10.1007/S13277-015-3483-2. Epub 2015 May 15. Microrna-142-3P Inhibits Cell Proliferation And Invasion Of Cervical Cancer Cells By Targeting Fzd7.
Li Q, Lv Ll, Wu M, Zhang Xl, Liu H, Liu Bc. J Cell Biochem. 2013 Mar;114(3):632-8. Doi: 10.1002/Jcb.24405. Dexamethasone Prevents Monocyte-Induced Tubular Epithelial-Mesenchymal Transition In Hk-2 Cells.
Tang R, Yang C, Tao Jl, You Yk, An N, Li Sm, Wu Hl, Liu Hf. Cell Biol Int. 2011 Sep;35(9):953-9. Doi: 10.1042/Cbi20100668. Epithelial-Mesenchymal Transdifferentiation Of Renal Tubular Epithelial Cells Induced By Urinary Proteins Requires The Activ...
Han P, Fu Y, Luo M, He J, Liu J, Liao J, Tian D, Yan W. Dig Dis Sci. 2014 May;59(5):992-1000. Doi: 10.1007/S10620-013-2992-3. Epub 2014 Jan 18. Bves Inhibition Triggers Epithelial-Mesenchymal Transition In Human Hepatocellular Carcinoma.
Duan F, Lin M, Li C, Ding X, Qian G, Zhang H, Ge S, Fan X, Li J. Cancer Biol Ther. 2014 May;15(5):544-59. Doi: 10.4161/Cbt.28157. Epub 2014 Mar 11. Effects Of Inhibition Of Hedgehog Signaling On Cell Growth And Migration Of Uveal Melanoma Cells.
Xia H, Cheung Wk, Sze J, Lu G, Jiang S, Yao H, Bian Xw, Poon Ws, Kung Hf, Lin Mc. J Biol Chem. 2010 Nov 19;285(47):36995-7004. Doi: 10.1074/Jbc.M110.133744. Epub 2010 Sep 7. Mir-200A Regulates Epithelial-Mesenchymal To Stem-Like Transition Via Zeb...
Li H, Chen L, Zeng S, Li X, Zhang X, Lin C, Zhang M, Xie S, He Y, Shu S, Yang L, Tang S, Fu X. Exp Cell Res. 2015 Mar 1;332(1):67-77. Doi: 10.1016/J.Yexcr.2015.01.014. Epub 2015 Jan 30. Matrigel Basement Membrane Matrix Induces Eccrine Sweat Gland...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to e-cadherin antibody, cdh1 antibody