Anti-Phospho-E2F-1 S364 Antibody

E2F-1 antibody

Boster Bio Anti-Phospho-E2F-1 S364 Antibody (Catalog # P00257). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse. Cited in 1 publication(s).

Product Info Summary

SKU: P00257
Size: 100ug
Reactive Species: Human, Mouse
Host: Rabbit
Application: ELISA, IHC, WB

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Product Name

Anti-Phospho-E2F-1 S364 Antibody

View all E2F-1 Antibodies

SKU/Catalog Number

P00257

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Phospho-E2F-1 S364 Antibody (Catalog # P00257). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Phospho-E2F-1 S364 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00257)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 350-375 of Human E2F-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P00257 is reactive to E2F1 in Human, Mouse

Applications

P00257 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

46.92kDa

Background of E2F-1

E2F-1 (also known as transcription factor E2F-1) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several domains conserved through evolution that are found in most members of the family. These domains include a DNA binding domain, a dimerization domain, a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and two other members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis as well as it can block adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. Anti-E2F-1 pS364 Antibody is useful for researchers involved in transcription factor and DNA binding research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:100,000
IHC: 2 mg/ml - 20 µg/ml
WB: 1:250 - 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For E2F1 (Source: Uniprot.org, NCBI)

Gene Name

E2F1

Full Name

Transcription factor E2F1

Weight

46.92kDa

Superfamily

E2F/DP family

Alternative Names

E2F transcription factor 1; E2F1; E2F-1; PBR3; PRB-binding protein E2F-1; RBAP1; RBAP-1; RBBP3; RBBP-3; RBP3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; transcription factor E2F1 E2F1 E2F-1, RBAP1, RBBP3, RBP3 E2F transcription factor 1 transcription factor E2F1|PBR3|PRB-binding protein E2F-1|RBAP-1|RBBP-3|retinoblastoma-associated protein 1|retinoblastoma-binding protein 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on E2F1, check out the E2F1 Infographic

E2F1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for E2F1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

P00257 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

GCN5 Potentiates Glioma Proliferation and Invasion via STAT3 and AKT Signaling Pathways

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5 Customer Q&As for Anti-Phospho-E2F-1 S364 Antibody

Question

Can you help my question with product P00257, anti-Phospho-E2F-1 S364 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-03-26

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00257 anti-Phospho-E2F-1 S364 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-26

Question

Do you have a BSA free version of anti-Phospho-E2F-1 S364 antibody P00257 available?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-E2F-1 S364 antibody P00257 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-13

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas skin using anti-Phospho-E2F-1 S364 antibody P00257. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-03

Question

I see that the anti-Phospho-E2F-1 S364 antibody P00257 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-07

Question

See attached the WB image, lot number and protocol we used for pancreas skin using anti-Phospho-E2F-1 S364 antibody P00257. Please let me know if you require anything else.

G. Parker

Verified customer

Asked: 2017-08-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-08-16

Order DetailsPrice
P00257

100 μg

$599

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