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SKU:PA1560
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-E2F1 Antibody
SKU/Catalog Number PA1560
Description Rabbit IgG polyclonal antibody for Transcription factor E2F1(E2F1) detection. Tested with WB in Human.
Cite This Product Anti-E2F1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1560)
Replacement Item This antibody may replace the following items: sc-130188|sc-137059|sc-137059-X|sc-137060|sc-137060-X|sc-137110|sc-137110-X|sc-1699|sc-1699-X|sc-18596|sc-18598|sc-193|sc-193-X|sc-22820|sc-22820-X|sc-251|sc-251-X|sc-25787|sc-271497|sc-271497-X|sc-376225|sc-390218|sc-56661|sc-56662 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human E2F1(246-260aa VTCQDLRSIADPAEQ), identical to the related mouse and rat sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name E2F1
Protein Name Transcription factor E2F1(E2F-1)
Molecular Weight 46920 MW
Protein Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812). .
Sequence Similarities Belongs to the E2F/DP family.
Subcellular Localization Nucleus.
Uniprot ID Q01094
Alternative Names Transcription factor E2F1;E2F-1;PBR3;Retinoblastoma-associated protein 1;RBAP-1;Retinoblastoma-binding protein 3;RBBP-3;pRB-binding protein E2F-1;E2F1;RBBP3;
Research Areas |epigenetics and nuclear signaling|transcription|domain families|forkhead box| epigenetics and nuclear signaling|other factors| cancer|oncoproteins/suppressors|tumor suppressors|rb family|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Transcription factor E2F1(E2F-1)

Transcription factor E2F1 is a protein that in humans is encoded by the E2F1 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family of transcription factors appears to play a critical role in the transcription of certain genes required for cell cycle progression. E2F1, the first cloned member of this family, is regulated during the cell cycle at the mRNA level by changes in transcription of the E2F1 gene and at the protein level by complex formation with proteins such as the retinoblastoma gene product(pRB), cyclin A and DP1. E2F1 can override a pRB-induced G1/S block and can behave as an oncogene in certain cells. E2F1 was cloned and was found to contain seven exons. Fluorescence in situ hybridization localized E2F1 to chromosome 20q11. The E2F1 transcription factor can promote proliferation or apoptosis when activated, and is a key downstream target of the retinoblastoma tumour suppressor protein(pRB).

Anti-E2F1 Antibody Images

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Anti-E2F1 Antibody
Anti-E2F1 antibody, PA1560, Western blotting
All lanes: Anti E2F1 (PA1560) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 47KD
Observed bind size: 60KD
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Publications

Wang Py, Li Yj, Zhang S, Li Zl, Yue Z, Xie N, Xie Sy. Mol Cell Biochem. 2010 Jun;339(1-2):163-71. Doi: 10.1007/S11010-009-0380-2. Epub 2010 Jan 5. Regulating A549 Cells Growth By Aso Inhibiting Mirna Expression.
Xie Sy, Li Yj, Wang Py, Jiao F, Zhang S, Zhang Wj. Oncol Rep. 2010 Jun;23(6):1693-700. Mirna-Regulated Expression Of Oncogenes And Tumor Suppressor Genes In The Cisplatin-Inhibited Growth Of K562 Cells.
Zhou R, Lu Z, Liu K, Guo J, Liu J, Zhou Y, Yang J, Mi M, Xu H. Curr Cancer Drug Targets. 2015;14(9):860-71. Platycodin D Induces Tumor Growth Arrest By Activating Foxo3A Expression In Prostate Cancer In Vitro And In Vivo.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.