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SKU:PA2185
Clonality:Polyclonal
Application:IHC-P, IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-EAAT1 Antibody
Description Rabbit IgG polyclonal antibody for Excitatory amino acid transporter 1(SLC1A3) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.
Cite This Product Anti-EAAT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2185)
Replacement Item This antibody may replace the following items: sc-15316|sc-7757|sc-7758 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human EAAT1(519-537aa MKKPYQLIAQDNETEKPID), different from the related rat and mouse sequences by three amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name SLC1A3
Protein Name Excitatory amino acid transporter 1
Molecular Weight 59572 MW
Protein Function Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
Tissue Specificity Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
Subcellular Localization Membrane; Multi-pass membrane protein.
Uniprot ID P43003
Alternative Names Excitatory amino acid transporter 1;Sodium-dependent glutamate/aspartate transporter 1;GLAST-1;Solute carrier family 1 member 3;SLC1A3;EAAT1, GLAST, GLAST1;
Research Areas |neuroscience|neurotransmitter|amino acids|glutamate| neuroscience|aspartate|transporters|cell type marker|glia marker|astrocyte marker| signal transduction|metabolism|energy metabolism| stem cells|neural stem cells|surface molecules|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Excitatory amino acid transporter 1

Solute carrier family 1(glial high-affinity glutamate transporter), member 3, also known as SLC1A3, EAAT1 or GLAST, is a protein that in humans is encoded by the SLC1A3 gene. This gene is a member of high affinity glutamate transporter family. SLC1A3 is mapped to chromosome 5p13.2 by fluorescence in situ hybridization(FISH). This gene transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. This gene acts as a symport by cotransporting sodium.

Anti-EAAT1 Antibody Images

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Anti-EAAT1 Antibody
Anti-EAAT1 antibody, PA2185, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Anti-EAAT1 Antibody
Anti-EAAT1 antibody, PA2185, IHC(P)
IHC(P): Rat Brain Tissue
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Publications

Ding Y, Zhang K, Liu S, Zhang Q, Ma C, Bruce Ic, Zhang X. Exp Ther Med. 2014 Dec;8(6):1909-1913. Epub 2014 Oct 15. Tumor Necrosis Factor-?? Promotes The Expression Of Excitatory Amino-Acid Transporter 2 In Astrocytes: Optimal Concentration And Incu...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to glast antibody, eaat1 antibody