|Application:||IHC-P, IHC-F, WB|
Data & Images
|Product Name||Anti-EAAT1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Excitatory amino acid transporter 1(SLC1A3) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.|
|Cite This Product||Anti-EAAT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2185)|
|Replacement Item||This antibody may replace the following items: sc-15316|sc-7757|sc-7758 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||IHC-P, IHC-F, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human EAAT1(519-537aa MKKPYQLIAQDNETEKPID), different from the related rat and mouse sequences by three amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Excitatory amino acid transporter 1|
|Molecular Weight||59572 MW|
|Protein Function||Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.|
|Tissue Specificity||Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.|
|Subcellular Localization||Membrane; Multi-pass membrane protein.|
|Alternative Names||Excitatory amino acid transporter 1;Sodium-dependent glutamate/aspartate transporter 1;GLAST-1;Solute carrier family 1 member 3;SLC1A3;EAAT1, GLAST, GLAST1;|
|Research Areas|||neuroscience|neurotransmitter|amino acids|glutamate| neuroscience|aspartate|transporters|cell type marker|glia marker|astrocyte marker| signal transduction|metabolism|energy metabolism| stem cells|neural stem cells|surface molecules||
Background for Excitatory amino acid transporter 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-EAAT1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, By Heat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, Human
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-EAAT1 Antibody Images
Click the images to enlarge.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
IHC(P): Rat Brain Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,