Anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal Antibody

EAAT2/GLT1 antibody

Boster Bio Anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal Antibody catalog # M01713. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: M01713
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, WB

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Product Name

Anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal Antibody

View all EAAT2/GLT1 Antibodies

SKU/Catalog Number

M01713

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal Antibody catalog # M01713. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01713)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ABED-19

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human EAAT2

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01713 is reactive to SLC1A2 in Human, Mouse, Rat

Applications

M01713 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

62.104kDa

Background of EAAT2/GLT1

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For SLC1A2 (Source: Uniprot.org, NCBI)

Gene Name

SLC1A2

Full Name

Excitatory amino acid transporter 2

Weight

62.104kDa

Superfamily

dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family

Alternative Names

EAAT2; GLT1; member 2; SLC1A2; solute carrier family 1 (glial high affinity glutamate transporter), member 2; Solute carrier family 1 member 2 SLC1A2 DEE41, EAAT2, EIEE41, GLT-1, HBGT solute carrier family 1 member 2 excitatory amino acid transporter 2|excitotoxic amino acid transporter 2|glutamate/aspartate transporter II|sodium-dependent glutamate/aspartate transporter 2|solute carrier family 1 (glial high affinity glutamate transporter), member 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SLC1A2, check out the SLC1A2 Infographic

SLC1A2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SLC1A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M01713 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Song T,Chen W,Chen X,Zhang H,Zou Y,Wu H,Lin F,Ren L,Kang Y,Lei H.Repeated fluoxetine treatment induces transient and long-term astrocytic plasticity in the medial prefrontal cortex of normal adult rats.Prog Neuropsychopharmacol Biol Psychiatry.2021 Jan 20
Species: Rat
M01713 usage in article: APP:WB, SAMPLE:MPFC TISSUE, DILUTION:1:500

Ding Y, Zhang K, Liu S, Zhang Q, Ma C, Bruce Ic, Zhang X. Exp Ther Med. 2014 Dec;8(6):1909-1913. Epub 2014 Oct 15. Tumor Necrosis Factor-?? Promotes The Expression Of Excitatory Amino-Acid Transporter 2 In Astrocytes: Optimal Concentration And Inc...

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10 Customer Q&As for Anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal Antibody

Question

I understand that the actual sequence is confidential. But please let me know the sequence is in intra- or extra-cellular domains?

Verified customer

Asked: 2022-07-19

Answer

Thank you for your questions. The immunogen sequence for M01713 is VAPPPDEEANATSA. It is in extracellular domain. Please feel free to contact us for any questions you have.

Boster Scientific Support

Answered: 2022-07-19

Question

According to your website, this is applicable to IP and WB. In WB, proteins are denatured. I want to make sure that the antibody binds to native protein (not denatured). Is this IP native or denatured proteins? Do you have any data on other applications, such as FCM, IHC, etc.?

Verified customer

Asked: 2022-07-19

Answer

Thank you for your inquiry. RIPA lysis buffer was used to prepare protein samples for IP. The proteins can be basically considered to have natural protein structure or close to native protein structure. Other applications such as FCM, IHC, etc. are not recommended.

Boster Scientific Support

Answered: 2022-07-19

Question

I am interested in to test anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody M01713 on human brain for research purposes, then I may be interested in using anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody M01713 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-18

Answer

The products we sell, including anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody M01713, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-18

Question

I have a customer who would like to reorder M01713. Do you have 5 vials of lot# 0011812m131? What is the expiration date?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

We have 5 vials of M01713 from lot#0011812M13120. Please write down the lot# on your order when you place it to us. We can only send them to you around January 22, 2018 due to spring festival holiday. Contact us freely for any other questions.

Boster Scientific Support

Answered: 2019-10-16

Question

Would anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody M01713 work for IP with brain?

G. Yang

Verified customer

Asked: 2019-01-04

Answer

According to the expression profile of brain, SLC1A2 is highly expressed in brain. So, it is likely that anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody M01713 will work for IP with brain.

Boster Scientific Support

Answered: 2019-01-04

Question

See below the WB image, lot number and protocol we used for brain using anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody M01713. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-07-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-07-16

Question

I see that the anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody M01713 works with IP, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-04-11

Answer

You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-04-11

Question

I was wanting to use your anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody for IP for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?

M. Bhatt

Verified customer

Asked: 2018-01-08

Answer

You can see on the product datasheet, M01713 anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-01-08

Question

Is this M01713 anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody reactive to the isotypes of SLC1A2?

Verified Customer

Verified customer

Asked: 2017-08-02

Answer

The immunogen of M01713 anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody is A synthesized peptide derived from human EAAT2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-08-02

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-EAAT2/GLT-1/SLC1A2 Rabbit Monoclonal antibody M01713. Let me know if you need anything else.

B. Kulkarni

Verified customer

Asked: 2014-04-11

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-04-11

Order DetailsPrice
M01713

100μL

$370
M01713-30ul

30μL sample

$99
M01713-carrier-free

Carrier Free

$370

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