Anti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband™

Rabbit IgG polyclonal antibody for EBP50/NHERF-1/SLC9A3R1 detection. Tested with WB, IHC-P, Direct ELISA in Human;Mouse;Rat.

SKU A02427-1
Size 100μg/vial
Reactivity Human, Mouse, Rat
Host Rabbit
Applications ELISA, IHC-P, WB

Overview

Product Name Anti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband™
See all SLC9A3R1 primary antibodies, ELISA kits and proteins
SKU/Catalog Number A02427-1
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for EBP50/NHERF-1/SLC9A3R1 detection. Tested with WB, IHC-P, Direct ELISA in Human;Mouse;Rat.
Cite This Product Anti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02427-1)
Specificity Anti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband™ (A02427-1) reacts with Human, Mouse, Rat SLC9A3R1, in native form and recombinant. Superfamily members of SLC9A3R1 are not reactive to A02427-1.
Host Rabbit
Contents/Buffer Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Form Lyophilized
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen E.coli-derived human EBP50/NHERF-1/SLC9A3R1 recombinant protein (Position: K69-R338).
Reactivity Human, Mouse, Rat

Assay Details

Our Boster Quality Guarantee for Anti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband™ covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband™ (A02427-1).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Images And Assay Conditions

/A/0/A02427-1-SLC9A3R1-primary-antibodies-IHC-testing-1.jpg

Figure 1. IHC analysis of SLC9A3R1 using anti-SLC9A3R1 antibody (A02427-1).
SLC9A3R1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC9A3R1 Antibody (A02427-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

/A/0/A02427-1-SLC9A3R1-primary-antibodies-IHC-testing-2.jpg

Figure 2. IHC analysis of SLC9A3R1 using anti-SLC9A3R1 antibody (A02427-1).
SLC9A3R1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC9A3R1 Antibody (A02427-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

/A/0/A02427-1-SLC9A3R1-primary-antibodies-IHC-testing-3.jpg

Figure 3. IHC analysis of SLC9A3R1 using anti-SLC9A3R1 antibody (A02427-1).
SLC9A3R1 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC9A3R1 Antibody (A02427-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

/A/0/A02427-1-SLC9A3R1-primary-antibodies-IHC-testing-4.jpg

Figure 4. IHC analysis of SLC9A3R1 using anti-SLC9A3R1 antibody (A02427-1).
SLC9A3R1 was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC9A3R1 Antibody (A02427-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

/A/0/A02427-1-SLC9A3R1-primary-antibodies-IHC-testing-5.jpg

Figure 5. IHC analysis of SLC9A3R1 using anti-SLC9A3R1 antibody (A02427-1).
SLC9A3R1 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC9A3R1 Antibody (A02427-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

/a/0/a02427-1-ebp50-primary-antibodies-wb-testing-6.jpg

Figure 6. Western blot analysis of SLC9A3R1 using anti-SLC9A3R1 antibody (A02427-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysate,
Lane 2: human HepG2 whole cell lysate,
Lane 3: human Caco-2 whole cell lysate,
Lane 4: human U-937 whole cell lysate,
Lane 5: human Hela whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC9A3R1 antigen affinity purified polyclonal antibody (Catalog # A02427-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC9A3R1 at approximately 50KD. The expected band size for SLC9A3R1 is at 39KD.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id O14745
Gene Name SLC9A3R1
Protein Name SLC9A3 regulator 1
Tissue Specificity Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
Alternative Names Na(+)/H(+) exchange regulatory cofactor NHE-RF1; NHERF-1; Ezrin-radixin-moesin-binding phosphoprotein 50; EBP50; Regulatory cofactor of Na(+)/H(+) exchanger; Sodium-hydrogen exchanger regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1; SLC9A3R1; NHERF; NHERF1
Subcellular Localization Apical cell membrane. Cytoplasm. Endomembrane system. Peripheral membrane protein. Filopodium. Ruffle. Microvillus.

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli. Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa.
Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.
Background Sodium-hydrogen antiporter 3 regulator 1 is a regulator of Sodium-hydrogen antiporter 3. It is encoded by the gene SLC9A3R1. It is mapped to 17q25.1. This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Publishing with Anti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband™ (A02427-1)? Please let us know so we can cite the reference in this product datasheet. Email us at [email protected]

Write Your Own Review
Only registered users can write reviews. Please Sign in or create an account
In stock
Order Product
A02427-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.

Write a review for A02427-1