Product Info Summary
SKU: | M02296 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-EEA1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02296
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-EEA1 Rabbit Monoclonal Antibody catalog # M02296. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EEA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02296)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ECH-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human EEA1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M02296 is reactive to EEA1 in Human, Mouse, Rat
Applications
M02296 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
38 kDa
Calculated molecular weight
162.466kDa
Background of EEA1
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of EEA1 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For EEA1 (Source: Uniprot.org, NCBI)
Gene Name
EEA1
Full Name
Early endosome antigen 1
Weight
162.466kDa
Alternative Names
early endosome antigen 1; early endosome antigen 1, 162kD; early endosome-associated protein; EEA1; Endosome-associated protein p162; MST105; MSTP105; ZFYVE2; ZFYVE2MST105; Zinc finger FYVE domain-containing protein 2 EEA1 MST105, MSTP105, ZFYVE2 early endosome antigen 1 early endosome antigen 1|early endosome antigen 1, 162kD|early endosome-associated protein|endosome-associated protein p162|zinc finger FYVE domain-containing protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EEA1, check out the EEA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EEA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EEA1 Rabbit Monoclonal Antibody (M02296)
Hello CJ!
M02296 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ji S,Dong W,Qi Y,Gao H,Zhao D,Xu M,Li T,Yu H,Sun Y,Ma R,Shi J,Gao C.Phagocytosis by endothelial cells inhibits procoagulant activity of platelets of essential thrombocythemia in vitro. J Thromb Haemost. 2020 Jan;18(1):222-233.doi: 10.1111/jth.14617.Epub 2
Species: Human
M02296 usage in article: APP:IF, SAMPLE:PLATELETS, DILUTION:NA
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-EEA1 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-EEA1 Rabbit Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
17 Customer Q&As for Anti-EEA1 Rabbit Monoclonal Antibody
Question
Will anti-EEA1 Rabbit Monoclonal antibody M02296 work on goat WB with fetal brain cortex?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
Our lab technicians have not tested anti-EEA1 Rabbit Monoclonal antibody M02296 on goat. You can run a BLAST between goat and the immunogen sequence of anti-EEA1 Rabbit Monoclonal antibody M02296 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat fetal brain cortex in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-21
Question
Will M02296 anti-EEA1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
It shows on the product datasheet, M02296 anti-EEA1 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-15
Question
We ordered your anti-EEA1 Rabbit Monoclonal antibody for IF on fetal brain cortex in the past. I am using mouse, and We intend to use the antibody for WB next. you antibody examining fetal brain cortex as well as biceps brachii in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
I took a look at the website and datasheets of our anti-EEA1 Rabbit Monoclonal antibody and I see that M02296 has been validated on mouse in both IF and WB. Thus M02296 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-02
Question
Here is the WB image, lot number and protocol we used for biceps brachii using anti-EEA1 Rabbit Monoclonal antibody M02296. Please let me know if you require anything else.
S. Anderson
Verified customer
Asked: 2019-12-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-18
Question
I was wanting to use your anti-EEA1 Rabbit Monoclonal antibody for IF for rat biceps brachii on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat biceps brachii identification?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
It shows on the product datasheet, M02296 anti-EEA1 Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat biceps brachii in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-02
Question
I see that the anti-EEA1 Rabbit Monoclonal antibody M02296 works with IF, what is the protocol used to produce the result images on the product page?
B. Li
Verified customer
Asked: 2019-11-18
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-18
Question
Can you help my question with product M02296, anti-EEA1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02296 anti-EEA1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-30
Question
I am looking for to test anti-EEA1 Rabbit Monoclonal antibody M02296 on rat biceps brachii for research purposes, then I may be interested in using anti-EEA1 Rabbit Monoclonal antibody M02296 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
The products we sell, including anti-EEA1 Rabbit Monoclonal antibody M02296, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-17
Question
Will anti-EEA1 Rabbit Monoclonal antibody M02296 work for IF with biceps brachii?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
According to the expression profile of biceps brachii, EEA1 is highly expressed in biceps brachii. So, it is likely that anti-EEA1 Rabbit Monoclonal antibody M02296 will work for IF with biceps brachii.
Boster Scientific Support
Answered: 2019-07-31
Question
My team were content with the WB result of your anti-EEA1 Rabbit Monoclonal antibody. However we have been able to see positive staining in cervix carcinoma erythroleukemia cytoplasm. early endosome membrane using this antibody. Is that expected? Could you tell me where is EEA1 supposed to be expressed?
K. Johnson
Verified customer
Asked: 2019-06-20
Answer
From literature, cervix carcinoma erythroleukemia does express EEA1. Generally EEA1 expresses in cytoplasm. early endosome membrane. Regarding which tissues have EEA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 7768953, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal brain cortex, Pubmed ID: 9697774
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-06-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for biceps brachii using anti-EEA1 Rabbit Monoclonal antibody M02296. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-11
Question
Do you have a BSA free version of anti-EEA1 Rabbit Monoclonal antibody M02296 available?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-EEA1 Rabbit Monoclonal antibody M02296 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-17
Question
you antibody using your anti-EEA1 Rabbit Monoclonal antibody for viral rna genome replication studies. Has this antibody been tested with western blotting on eea1in hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-01
Answer
We appreciate your inquiry. This M02296 anti-EEA1 Rabbit Monoclonal antibody is tested on eea1in hela cells. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-01
Question
Is this M02296 anti-EEA1 Rabbit Monoclonal antibody reactive to the isotypes of EEA1?
R. Williams
Verified customer
Asked: 2019-04-08
Answer
The immunogen of M02296 anti-EEA1 Rabbit Monoclonal antibody is A synthesized peptide derived from human EEA1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-08
Question
We are currently using anti-EEA1 Rabbit Monoclonal antibody M02296 for mouse tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
The anti-EEA1 Rabbit Monoclonal antibody (M02296) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-08
Question
Is a blocking peptide available for product anti-EEA1 Rabbit Monoclonal antibody (M02296)?
Verified Customer
Verified customer
Asked: 2018-01-19
Answer
We do provide the blocking peptide for product anti-EEA1 Rabbit Monoclonal antibody (M02296). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-19
Question
We have been able to see staining in mouse cervix carcinoma. Are there any suggestions? Is anti-EEA1 Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
B. Kulkarni
Verified customer
Asked: 2013-04-30
Answer
From what I have seen in literature cervix carcinoma does express EEA1. From what I have seen in Uniprot.org, EEA1 is expressed in biceps brachii, cervix carcinoma, fetal brain cortex, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have EEA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 7768953, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal brain cortex, Pubmed ID: 9697774
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2013-04-30