Product Info Summary
SKU: | M00830 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-EF-2 EEF2 Monoclonal Antibody
SKU/Catalog Number
M00830
Size
100ul
Form
Liquid
Description
Boster Bio Anti-EF-2 EEF2 Monoclonal Antibody catalog # M00830. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EF-2 EEF2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00830)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
5B6
Isotype
IgG
Immunogen
Purified recombinant fragment of human EF-2 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M00830 is reactive to EEF2 in Human
Applications
M00830 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
95.338kDa
Background of eEF-2
TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 4. ELISA validation of EEF2 using Anti-EF-2 EEF2 Monoclonal Antibody (M00830).
ELISA analysis of EF-2 antibody.Click image to see more details
Figure 3. Immunofluorescent staining data of EEF2 using Anti-EF-2 EEF2 Monoclonal Antibody (M00830).
Immunofluorescence (IF) analysis of 3T3-L1 cells using EF-2 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Click image to see more details
Figure 2. Immunohistochemistry validation of EEF2 using Anti-EF-2 EEF2 Monoclonal Antibody (M00830).
Immunohistochemistry (IHC) analysis of paraffin-embedded liver cancer tissues (left) and kidney cancer tissues (right) with DAB staining using EF-2 Monoclonal Antibody.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-EF-2 EEF2 Monoclonal Antibody M00830
Western Blot (WB) analysis using EF-2 Monoclonal Antibody against HepG2 cell lysate.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For EEF2 (Source: Uniprot.org, NCBI)
Gene Name
EEF2
Full Name
Elongation factor 2
Weight
95.338kDa
Superfamily
TRAFAC class translation factor GTPase superfamily
Alternative Names
eEF2; eEF-2; EF-2; EF2EEF-2; elongation factor 2; eukaryotic translation elongation factor 2; polypeptidyl-tRNA translocase EEF2 EEF-2, EF-2, EF2, SCA26 eukaryotic translation elongation factor 2 elongation factor 2|epididymis secretory sperm binding protein|polypeptidyl-tRNA translocase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EEF2, check out the EEF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EEF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EF-2 EEF2 Monoclonal Antibody (M00830)
Hello CJ!
No publications found for M00830
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-EF-2 EEF2 Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-EF-2 EEF2 Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question