Anti-Pro-epidermal growth factor EGF Antibody

Boster Bio Anti-Pro-epidermal growth factor EGF Antibody catalog # A00378. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A00378
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Pro-epidermal growth factor EGF Antibody

See all EGF products

SKU/Catalog Number

A00378

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Pro-epidermal growth factor EGF Antibody catalog # A00378. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Pro-epidermal growth factor EGF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00378)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human EGF.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00378 is reactive to EGF in Human, Mouse, Rat

Applications

A00378 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For EGF (Source: Uniprot.Org, NCBI)

Uniprot ID

P01133

Gene ID

1950

Gene Name

EGF

Full Name

Pro-epidermal growth factor

Weight

133.994kDa

Alternative Names

beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone EGF HOMG4, URG epidermal growth factor pro-epidermal growth factor|beta-urogastrone

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on EGF, check out the EGF Infographic

EGF infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for EGF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00378 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effect of IL-10 on the expression of HSC growth factors in hepatic fibrosis rat

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15 Customer Q&As for Anti-Pro-epidermal growth factor EGF Antibody

Question

Will anti-EGF antibody A00378 work for WB with teratocarcinoma?

Verified Customer

Verified customer

Asked: 2020-03-10

Answer

According to the expression profile of teratocarcinoma, EGF is highly expressed in teratocarcinoma. So, it is likely that anti-EGF antibody A00378 will work for WB with teratocarcinoma.

Boster Scientific Support

Answered: 2020-03-10

Question

We are currently using anti-EGF antibody A00378 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

The anti-EGF antibody (A00378) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-14

Question

I was wanting to use using your anti-EGF antibody for peptidyl-tyrosine phosphorylation studies. Has this antibody been tested with western blotting on 3t3 cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-01-16

Answer

We appreciate your inquiry. This A00378 anti-EGF antibody is tested on 3t3 cells. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-01-16

Question

Will A00378 anti-EGF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

It shows on the product datasheet, A00378 anti-EGF antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-06

Question

I was wanting to use your anti-EGF antibody for WB for rat teratocarcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat teratocarcinoma identification?

Verified Customer

Verified customer

Asked: 2019-10-24

Answer

You can see on the product datasheet, A00378 anti-EGF antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat teratocarcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-24

Question

My team were happy with the WB result of your anti-EGF antibody. However we have seen positive staining in teratocarcinoma membrane using this antibody. Is that expected? Could you tell me where is EGF supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-08-01

Answer

From what I have seen in literature, teratocarcinoma does express EGF. Generally EGF expresses in membrane. Regarding which tissues have EGF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Kidney, Pubmed ID: 3491360
Teratocarcinoma, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-08-01

Question

Is a blocking peptide available for product anti-EGF antibody (A00378)?

Verified Customer

Verified customer

Asked: 2019-07-26

Answer

We do provide the blocking peptide for product anti-EGF antibody (A00378). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-26

Question

I was wanting to use to test anti-EGF antibody A00378 on rat teratocarcinoma for research purposes, then I may be interested in using anti-EGF antibody A00378 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-07-11

Answer

The products we sell, including anti-EGF antibody A00378, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-07-11

Question

See attached the WB image, lot number and protocol we used for teratocarcinoma using anti-EGF antibody A00378. Please let me know if you require anything else.

M. Gonzalez

Verified customer

Asked: 2019-06-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-21

Question

Do you have a BSA free version of anti-EGF antibody A00378 available?

M. Wu

Verified customer

Asked: 2018-07-12

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-EGF antibody A00378 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-07-12

Question

We have seen staining in mouse renal medulla. Any tips? Is anti-EGF antibody supposed to stain renal medulla positively?

Verified Customer

Verified customer

Asked: 2018-02-08

Answer

From what I have seen in literature renal medulla does express EGF. From what I have seen in Uniprot.org, EGF is expressed in renal medulla, kidney, teratocarcinoma, colon, among other tissues. Regarding which tissues have EGF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Kidney, Pubmed ID: 3491360
Teratocarcinoma, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-02-08

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for teratocarcinoma using anti-EGF antibody A00378. Let me know if you need anything else.

B. Carter

Verified customer

Asked: 2017-10-31

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-10-31

Question

Is this A00378 anti-EGF antibody reactive to the isotypes of EGF?

M. Rodriguez

Verified customer

Asked: 2017-01-25

Answer

The immunogen of A00378 anti-EGF antibody is Synthesized peptide derived from the Internal region of human EGF. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-01-25

Question

Can you help my question with product A00378, anti-EGF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

B. Lewis

Verified customer

Asked: 2015-11-02

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00378 anti-EGF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-11-02

Question

I see that the anti-EGF antibody A00378 works with WB, what is the protocol used to produce the result images on the product page?

A. Evans

Verified customer

Asked: 2014-10-03

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2014-10-03

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A00378
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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