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SKU:PB9016
Clonality:Polyclonal
Application:WB
Price: $240.00
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Overview

Product Name Anti-EGFR Picoband™ Antibody
Description Rabbit IgG polyclonal antibody for Epidermal growth factor receptor(EGFR) detection. Tested with WB in Human;Rat.
Cite This Product Anti-EGFR Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9016)
Replacement Item This antibody may replace the following items: sc-165701|sc-382346|sc-398145 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Rat
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2016!
Immunogen E.coli-derived human EGFR recombinant protein (Position: L25-K346). Human EGFR shares 89% amino acid (aa) sequence identity with mouse EGFR.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name EGFR
Protein Name Epidermal growth factor receptor
Gene Full Name Epidermal growth factor receptor
Protein Function Receptor tyrosine kinase binding ligands of the EGFfamily and activating several signaling cascades to convertextracellular cues into appropriate cellular responses. Knownligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN,BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF.Ligand binding triggers receptor homo- and/or heterodimerizationand autophosphorylation on key cytoplasmic residues. Thephosphorylated receptor recruits adapter proteins like GRB2 whichin turn activates complex downstream signaling cascades. Activatesat least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Mayalso activate the NF-kappa-B signaling cascade. Also directlyphosphorylates other proteins like RGS16, activating its GTPaseactivity and probably coupling the EGF receptor signaling to the Gprotein-coupled receptor signaling. Also phosphorylates MUC1 andincreases its interaction with SRC and CTNNB1/beta-catenin.
Tissue Specificity Ubiquitously expressed. Isoform 2 is alsoexpressed in ovarian cancers..
Sequence Similarities Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Subcellular Localization Cell membrane; In response toEGF, translocated from the cell membrane to the nucleus via Golgiand ER. Endocytosed upon activation by ligand. Colocalized withGPER1 in the nucleus of estrogen agonist-induced cancer-associatedfibroblasts (CAF).
Uniprot ID EGFR: P00533
Alternative Names Epidermal growth factor receptor; 2.7.10.1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR; ERBB, ERBB1, HER1;
Research Areas signal transduction|protein phosphorylation|tyrosine kinases|receptor tyrosine kinases|growth factors/hormones|egf|cancer|oncoproteins/suppressors|oncoproteins|growth factor receptors|tumor biomarkers|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Epidermal growth factor receptor

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.

Anti-EGFR Picoband™ Antibody Images

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Anti-EGFR Picoband™ Antibody
Anti-EGFR Picoband antibody, PB9016-1.jpg
All lanes: Anti-EGFR(PB9016) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: A431 Whole Cell Lysate at 40ug
Predicted bind size: 134KD
Observed bind size: 200KD

Customer Reviews

Review by Elena Deryugina
Quality

Source: Biocompare.com

"I would highly recommend this Boster antibody..."

--Elena Deryugina, CELL AND MOLECULAR BIOLOGY, THE SCRIPPS RESEARCH INSTITUTE, ASSISTANT PROFESSOR

Applications Western Blot
Sample Recombinant N-Terminal domain of human EGFR (aa 1-621) tested at 100 ng/lane
Detection SuperSignal West Pico Chemiluminescent Substrate

"Rabbit antibody PB9016 from Boster was probed for western blot detection of the recombinant N-terminal domain of human EGFR (100 ng/lane). The antibody nicely detected the protein band with the expected mol.wt. of recombinant protein. The antibody appears to be specific since non-specific bands were not detected in parallel lanes with cell lysates lacking EGFR. I would highly recommend this Boster antibody for detection of EGFR with the intact N-terminal domain."

(Posted on 8/23/2016)
Write a review for PB9016

Publications

Gu Y, Zhang S, Wu Q, Xu S, Cui Y, Yang Z, Zhao X, Sun B. J Exp Clin Cancer Res. 2010 Jan 22;29:6. Doi: 10.1186/1756-9966-29-6. Differential Expression Of Decorin, Egfr And Cyclin D1 During Mammary Gland Carcinogenesis In Ta2 Mice With Spontaneous ...
Wang Sb, Cheng Yn, Cui Sx, Zhong Jl, Ward Sg, Sun Lr, Chen Mh, Kokudo N, Tang W, Qu Xj. Clin Exp Metastasis. 2009;26(5):469-77. Doi: 10.1007/S10585-009-9246-Y. Epub 2009 Mar 5. Des-Gamma-Carboxy Prothrombin Stimulates Human Vascular Endothelial Ce...
Yue P, Gao Zh, Xue X, Cui Sx, Zhao Cr, Yuan Y, Yin Z, Inagaki Y, Kokudo N, Tang W, Qu Xj. Eur J Cancer. 2011 May;47(7):1115-24. Doi: 10.1016/J.Ejca.2011.01.017. Epub 2011 Feb 23. Des-??-Carboxyl Prothrombin Induces Matrix Metalloproteinase Activity...
Ge C, Yu M, Petitte Jn, Zhang C. Biol Reprod. 2009 Mar;80(3):528-36. Doi: 10.1095/Biolreprod.108.072728. Epub 2008 Nov 12. Epidermal Growth Factor-Induced Proliferation Of Chicken Primordial Germ Cells: Involvement Of Calcium/Protein Kinase C And ...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to egfr antibody, epidermal growth factor receptor antibody, mena antibody