Data & Images
|Product Name||Anti-EIF2AK1/Hri Antibody|
|Description||Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 2-alpha kinase 1(EIF2AK1) detection. Tested with WB, ICC in Human.|
|Cite This Product||Anti-EIF2AK1/Hri Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2024)|
|Replacement Item||This antibody may replace the following items: sc-80648|sc-18619|sc-18620|sc-28823|sc-133168|sc-166680|sc-23886|sc-71075|sc-136522|sc-136522-X|sc-133168|sc-136522|sc-136522-X|sc-166680|sc-18619|sc-18620|sc-23886|sc-28823|sc-71075|sc-80648 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human EIF2AK1(466-483aa CTDILQKNTDWTNRNGKR).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Eukaryotic translation initiation factor 2-alpha kinase 1|
|Molecular Weight||71106 MW|
|Protein Function||Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser- 48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions (By similarity). .|
|Tissue Specificity||Expressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level). .|
|Sequence Similarities||Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.|
|Subcellular Localization||Cytoplasm .|
|Alternative Names||Eukaryotic translation initiation factor 2-alpha kinase 1;126.96.36.199;Heme-controlled repressor;HCR;Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase;Heme-regulated inhibitor;Hemin-sensitive initiation factor 2-alpha kinase;EIF2AK1;HRI, KIAA1369;PRO1362;|
|Research Areas|||epigenetics and nuclear signaling|dna / rna|translation|regulation| signal transduction|protein phosphorylation|ser / thr kinases|s6k family| epigenetics and nuclear signaling|rnai|eukaryotic initiation factors (eif's)||
Background for Eukaryotic translation initiation factor 2-alpha kinase 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-EIF2AK1/Hri Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, -|
Immunocytochemistry , 0.5-1μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-EIF2AK1/Hri Antibody Images
Click the images to enlarge.
Recombinant Protein Detection Source: E.coli derived -recombinant Human EIF2AK1, 35.5KD (162aa tag+ R398-K551)
Lane 1: Recombinant Human EIF2AK1 Protein 10ng
Lane 2: Recombinant Human EIF2AK1 Protein 5ng
Lane 3: Recombinant Human EIF2AK1 Protein 2.5ng
ICC: HELA Cell
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,