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SKU:PA2024
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:ICC, WB
Price: $200.00
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Overview

Product Name Anti-EIF2AK1/Hri Antibody
SKU/Catalog Number PA2024
Description Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 2-alpha kinase 1(EIF2AK1) detection. Tested with WB, ICC in Human.
Cite This Product Anti-EIF2AK1/Hri Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2024)
Replacement Item This antibody may replace the following items: sc-80648|sc-18619|sc-18620|sc-28823|sc-133168|sc-166680|sc-23886|sc-71075|sc-136522|sc-136522-X|sc-133168|sc-136522|sc-136522-X|sc-166680|sc-18619|sc-18620|sc-23886|sc-28823|sc-71075|sc-80648 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human EIF2AK1(466-483aa CTDILQKNTDWTNRNGKR).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name EIF2AK1
Protein Name Eukaryotic translation initiation factor 2-alpha kinase 1
Molecular Weight 71106 MW
Protein Function Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser- 48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions (By similarity). .
Tissue Specificity Expressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level). .
Sequence Similarities Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Subcellular Localization Cytoplasm .
Uniprot ID Q9BQI3
Alternative Names Eukaryotic translation initiation factor 2-alpha kinase 1;2.7.11.1;Heme-controlled repressor;HCR;Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase;Heme-regulated inhibitor;Hemin-sensitive initiation factor 2-alpha kinase;EIF2AK1;HRI, KIAA1369;PRO1362;
Research Areas |epigenetics and nuclear signaling|dna / rna|translation|regulation| signal transduction|protein phosphorylation|ser / thr kinases|s6k family| epigenetics and nuclear signaling|rnai|eukaryotic initiation factors (eif's)|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Eukaryotic translation initiation factor 2-alpha kinase 1

EIF2AK1(Eukaryotic Translation Initiation Factor 2-Alpha Kinase 1), also called HRI, is an enzyme that in humans is encoded by the EIF2AK1 gene. Hartz(2010) mapped the EIF2AK1 gene to chromosome 7p22.1 based on an alignment of the EIF2AK1 sequence with the genomic sequence. Chen and London(1995) reviewed the activation and control of rat and rabbit Hri. Hri functions as a dimer, and its activation involves autophosphorylation, which requires an invariant lysine in kinase domain II. Using cDNA arrays and Northern blot analysis, Hwang et al.(2000) found that HRI was downregulated in the majority of ovarian cancers examined compared with normal ovarian tissues.

Anti-EIF2AK1/Hri Antibody Images

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Anti-EIF2AK1/Hri Antibody
Anti-EIF2AK1 antibody, PA2024, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human EIF2AK1, 35.5KD (162aa tag+ R398-K551)
Lane 1: Recombinant Human EIF2AK1 Protein 10ng
Lane 2: Recombinant Human EIF2AK1 Protein 5ng
Lane 3: Recombinant Human EIF2AK1 Protein 2.5ng
Anti-EIF2AK1/Hri Antibody
Anti-EIF2AK1 antibody, PA2024, ICC
ICC: HELA Cell
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to eif2ak1 antibody, hri antibody