Anti-EIF2S1 Antibody

eIF2 alpha/EIF2S1 antibody

Boster Bio Anti-EIF2S1 Antibody catalog # PA2029. Tested in IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PA2029
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-EIF2S1 Antibody

View all eIF2 alpha/EIF2S1 Antibodies

SKU/Catalog Number

PA2029

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-EIF2S1 Antibody catalog # PA2029. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EIF2S1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2029)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human EIF2S1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2029 is reactive to EIF2S1 in Human

Applications

PA2029 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

94 kDa

Calculated molecular weight

36.112kDa

Background of eIF2 alpha/EIF2S1

EIF2S1 (Eukaryotic Translation Initiation Factor 2, Subunit 1), also called EIF2-alpha, is a protein that in humans is encoded by the EIF2S1 gene. Hartz (2010) mapped the EIF2S1 gene to chromosome 14q23.3 based on an alignment of the EIF2S1 sequence with the genomic sequence (GRCh37). Ernst et al. (1987) stated that protein synthesis is inhibited due to phosphorylation of Eif2-alpha in hemin-deprived rabbit reticulocyte lysates. HeLa cells subjected to heat shock, serum deprivation, or interferon treatment followed by virus infection also show a correlation between EIF2-alpha phosphorylation and translational repression. Jacob et al. (1989) found that the alpha-Pal transcription factor bound to 2 palindromic sites within the EIF2-alpha promoter and was essential for transcription of the EIF2-alpha gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For EIF2S1 (Source: Uniprot.org, NCBI)

Gene Name

EIF2S1

Full Name

Eukaryotic translation initiation factor 2 subunit 1

Weight

36.112kDa

Superfamily

eIF-2-alpha family

Alternative Names

eIF2 alpha; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; EIF2S1 eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; EIF2S1 EIF2S1 EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A eukaryotic translation initiation factor 2 subunit alpha eukaryotic translation initiation factor 2 subunit 1|eIF-2-alpha|eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EIF2S1, check out the EIF2S1 Infographic

EIF2S1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EIF2S1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-EIF2S1 Antibody

Question

Would anti-EIF2S1 antibody PA2029 work for WB with cervix carcinoma erythroleukemia?

J. Mitchell

Verified customer

Asked: 2020-05-01

Answer

According to the expression profile of cervix carcinoma erythroleukemia, EIF2S1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-EIF2S1 antibody PA2029 will work for WB with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2020-05-01

Question

See attached the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-EIF2S1 antibody PA2029. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-04

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-04

Question

Is this PA2029 anti-EIF2S1 antibody reactive to the isotypes of EIF2S1?

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

The immunogen of PA2029 anti-EIF2S1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human EIF2S1(123-137aa KDEQLESLFQRTAWV). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-08

Question

We are currently using anti-EIF2S1 antibody PA2029 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-12

Answer

The anti-EIF2S1 antibody (PA2029) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-12

Question

Is a blocking peptide available for product anti-EIF2S1 antibody (PA2029)?

M. Carter

Verified customer

Asked: 2019-05-17

Answer

We do provide the blocking peptide for product anti-EIF2S1 antibody (PA2029). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-05-17

Question

Does PA2029 anti-EIF2S1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-11-15

Answer

As indicated on the product datasheet, PA2029 anti-EIF2S1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-11-15

Question

I was wanting to use your anti-EIF2S1 antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?

Verified Customer

Verified customer

Asked: 2018-02-05

Answer

As indicated on the product datasheet, PA2029 anti-EIF2S1 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-02-05

Order DetailsPrice
PA2029

100μg

$370
PA2029-10ug

10μg sample (liquid)

$99
PA2029-Biotin

100 μg Biotin conjugated

$570
PA2029-Cy3

100 μg Cy3 conjugated

$570
PA2029-Dylight488

100 μg Dylight488 conjugated

$570
PA2029-Dylight550

100 μg Dylight550 conjugated

$570
PA2029-Dylight594

100 μg Dylight594 conjugated

$570
PA2029-FITC

100 μg FITC conjugated

$570
PA2029-HRP

100 μg HRP conjugated

$570
PA2029-APC

100 μg APC conjugated

$670
PA2029-PE

100 μg PE conjugated

$670
PA2029-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PA2029
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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