Data & Images
|Product Name||Anti-EIF2S1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 2 subunit 1(EIF2S1) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-EIF2S1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2029)|
|Replacement Item||This antibody may replace the following items: sc-101670|sc-11386|sc-12412|sc-12412-R|sc-133132|sc-133227|sc-293100|sc-30882 from Santa Cruz Biotechnology.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human EIF2S1(123-137aa KDEQLESLFQRTAWV).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Eukaryotic translation initiation factor 2 subunit 1|
|Molecular Weight||36112 MW|
|Protein Function||Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.|
|Sequence Similarities||Belongs to the eIF-2-alpha family.|
|Subcellular Localization||Cytoplasmic granule . The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). .|
|Alternative Names||Eukaryotic translation initiation factor 2 subunit 1;Eukaryotic translation initiation factor 2 subunit alpha;eIF-2-alpha;eIF-2A;eIF-2alpha;EIF2S1;EIF2A;|
|Research Areas|||epigenetics and nuclear signaling|dna / rna|translation|regulation| epigenetics and nuclear signaling|rna processing|rnai|eukaryotic initiation factors (eif's)||
Background for Eukaryotic translation initiation factor 2 subunit 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-EIF2S1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-EIF2S1 Antibody Images
Click the images to enlarge.
Lane 1: COLO320 Cell Lysate
Lane 2: CEM Cell Lysate
Lane 3: RAJI Cell Lysate
Lane 4: HT1080 Cell Lysate
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Placenta Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,