Anti-EIF4A3 Antibody

EIF4A3 antibody

Boster Bio Anti-EIF4A3 Antibody catalog # A03095. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.

Product Info Summary

SKU: A03095
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-EIF4A3 Antibody

View all EIF4A3 Antibodies

SKU/Catalog Number

A03095

Size

100ul

Form

Liquid

Description

Boster Bio Anti-EIF4A3 Antibody catalog # A03095. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EIF4A3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03095)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human SPON1 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03095 is reactive to EIF4A3 in Human, Mouse, Rat

Applications

A03095 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

46.871kDa

Background of EIF4A3

Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity.

McGuinness B.E., PLoS Biol. 3:433-449(2005).
Kitajima T.S., Curr. Biol. 15:353-359(2005).
Tang Z., Proc. Natl. Acad. Sci. U.S.A. 101:18012-18017(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000-1:3000
IHC: 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For EIF4A3 (Source: Uniprot.org, NCBI)

Gene Name

EIF4A3

Full Name

Eukaryotic initiation factor 4A-III

Weight

46.871kDa

Superfamily

DEAD box helicase family

Alternative Names

ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DDX48; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; DKFZp686O16189; EC 3.6.1; EC 3.6.4.13; EIF4AIII; eIF-4A-III; eIF4A-III; eukaryotic initiation factor 4A-III; Eukaryotic initiation factor 4A-like NUK-34; Eukaryotic translation initiation factor 4A isoform 3; eukaryotic translation initiation factor 4A; eukaryotic translation initiation factor 4A3; hNMP 265; KIAA0111eukaryotic translation initiation factor 4A, isoform 3; MGC10862; NMP 265; NMP265; Nuclear matrix protein 265; NUK34 EIF4A3 DDX48, Fal1, MUK34, NMP265, NUK34, RCPS, eIF-4A-III, eIF4A-III, eIF4AIII eukaryotic translation initiation factor 4A3 eukaryotic initiation factor 4A-III|ATP-dependent RNA helicase DDX48|ATP-dependent RNA helicase eIF4A-3|DEAD (Asp-Glu-Ala-Asp) box polypeptide 48|DEAD box protein 48|NMP 265|eukaryotic initiation factor 4A-like NUK-34|nuclear matrix protein 265

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EIF4A3, check out the EIF4A3 Infographic

EIF4A3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EIF4A3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A03095-100ul

100ul

$405

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