|Application:||IHC-P, IHC-F, WB|
Data & Images
|Product Name||Anti-EIF6 Antibody|
|Description||Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 6(EIF6) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.|
|Cite This Product||Anti-EIF6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2031)|
|Replacement Item||This antibody may replace the following items: sc-70270|sc-70268|sc-366167|sc-390432|sc-390441 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC-P, IHC-F, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human EIF6 (82-96aa QHIRNSLPDTVQIRR), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Eukaryotic translation initiation factor 6|
|Molecular Weight||26599 MW|
|Protein Function||Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity. .|
|Tissue Specificity||Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). .|
|Subcellular Localization||Cytoplasm. Nucleus, nucleolus. Shuttles between cytoplasm and nucleus/nucleolus.|
|Alternative Names||Eukaryotic translation initiation factor 6 ;eIF-6 ;B(2)GCN homolog;B4 integrin interactor;CAB;p27(BBP);EIF6 ;EIF3A, ITGB4BP ;OK/SW-cl.27;|
|Research Areas|||neuroscience|cell adhesion proteins|receptors|integrins|beta integrins| epigenetics and nuclear signaling|transcription|mediator complex||
Background for Eukaryotic translation initiation factor 6
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-EIF6 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-EIF6 Antibody Images
Click the images to enlarge.
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: COLO320 Cell Lysate
Lane 4: SW620 Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: 293T Cell Lysate
Lane 7: HEPA Cell Lysate
IHC(P): Human Intestinal Cancer Tissue
IHC(F): Human Placenta Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,