Product Info Summary
SKU: | P01426 |
---|---|
Size: | 100 µl |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Phospho-Elk-1 (Ser383) Antibody
SKU/Catalog Number
P01426
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Elk-1 (Ser383) Antibody (Catalog # P01426). Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-Elk-1 (Ser383) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01426)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Clone Number
608
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 of human Elk-1, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P01426 is reactive to ELK1 in Mouse, Rat
Applications
P01426 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
44.888kDa
Background of Elk-1
Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors (Hill and Treisman, 1995). Elk-1 is thought to be phosphorylated by MAP kinase at Ser-383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1 (Li et al., 2003). Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus (Thiels et al., 2002).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
Click image to see more details
Western blot of recombinant Elk-1 showing specific immunolabeling of the ~46 kDa Elk-1 phosphorylated at Ser383 in the right lane (-). Phosphospecificity is shown in the left lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Protein Target Info & Infographic
Gene/Protein Information For ELK1 (Source: Uniprot.org, NCBI)
Gene Name
ELK1
Full Name
ETS domain-containing protein Elk-1
Weight
44.888kDa
Superfamily
ETS family
Alternative Names
Elk1; Elk-1; ELK1, member of ETS oncogene family; ETS domain-containing protein Elk-1; ETS-like gene 1 ELK1 ETS transcription factor ELK1 ETS domain-containing protein Elk-1|ELK1, ETS transcription factor|ELK1, member of ETS oncogene family|ETS-like gene 1|tyrosine kinase (ELK1) oncogene
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ELK1, check out the ELK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ELK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Elk-1 (Ser383) Antibody (P01426)
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No publications found for P01426
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Phospho-Elk-1 (Ser383) Antibody
Question
We are currently using anti-Phospho-Elk-1 (Ser383) antibody P01426 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
The anti-Phospho-Elk-1 (Ser383) antibody (P01426) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-15