Product Info Summary
SKU: | A08546 |
---|---|
Size: | 100μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-EMR2 ADGRE2 Antibody
SKU/Catalog Number
A08546
Size
100μl
Form
Liquid
Description
Boster Bio Anti-EMR2 ADGRE2 Antibody catalog # A08546. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EMR2 ADGRE2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08546)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human EMR2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08546 is reactive to ADGRE2 in Human
Applications
A08546 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
90.472kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC-P, 1:100-1:300
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of MCF7, PC12 cells using EMR2 Polyclonal antibody.
Click image to see more details
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Kidney, antibody was diluted at 1:200.
Protein Target Info & Infographic
Gene/Protein Information For ADGRE2 (Source: Uniprot.org, NCBI)
Gene Name
ADGRE2
Full Name
Adhesion G protein-coupled receptor E2
Weight
90.472kDa
Superfamily
G-protein coupled receptor 2 family
Alternative Names
CD312; DKFZp781B135; egf-like module containing, mucin-like, hormone receptor-like 2; egf-like module containing, mucin-like, hormone receptor-like sequence 2; EGF-like module receptor 2; EGF-like module-containing mucin-like hormone receptor-like 2; EMR2 ADGRE2 CD312, CD97, EMR2, VBU adhesion G protein-coupled receptor E2 adhesion G protein-coupled receptor E2|CD97 antigen|EGF-like module-containing mucin-like hormone receptor-like 2|Leukocyte antigen CD97|egf-like module containing, mucin-like, hormone receptor-like 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADGRE2, check out the ADGRE2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADGRE2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EMR2 ADGRE2 Antibody (A08546)
Hello CJ!
No publications found for A08546
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-EMR2 ADGRE2 Antibody
Question
I have a question about product A08546, anti-EMR2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08546 anti-EMR2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-02
Question
Will anti-EMR2 antibody A08546 work on monkey IHC with blood?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
Our lab technicians have not tested anti-EMR2 antibody A08546 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-EMR2 antibody A08546 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey blood in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-20
Question
Does A08546 anti-EMR2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-16
Answer
It shows on the product datasheet, A08546 anti-EMR2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-16
Question
I was wanting to use your anti-EMR2 antibody for IHC for human blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human blood identification?
J. Kulkarni
Verified customer
Asked: 2016-09-14
Answer
You can see on the product datasheet, A08546 anti-EMR2 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-09-14
Question
Here is the WB image, lot number and protocol we used for blood using anti-EMR2 antibody A08546. Please let me know if you require anything else.
C. Gonzalez
Verified customer
Asked: 2015-02-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-02-18
Question
I see that the anti-EMR2 antibody A08546 works with IHC, what is the protocol used to produce the result images on the product page?
G. Taylor
Verified customer
Asked: 2013-09-19
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-09-19