Anti-Endoglin / CD105 (Angiogenesis Marker) ENG Monoclonal Antibody

Endoglin/CD105 antibody

Boster Bio Anti-Endoglin / CD105 (Angiogenesis Marker) ENG Monoclonal Antibody (Catalog # M02997). Tested in Flow Cytometry, IF, WB applications. This antibody reacts with Human. Cited in 4 publication(s).

Product Info Summary

SKU: M02997
Size: 100ug/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IF, WB

Product Name

Anti-Endoglin / CD105 (Angiogenesis Marker) ENG Monoclonal Antibody

View all Endoglin/CD105 Antibodies

SKU/Catalog Number

M02997

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Endoglin / CD105 (Angiogenesis Marker) ENG Monoclonal Antibody (Catalog # M02997). Tested in Flow Cytometry, IF, WB applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Endoglin / CD105 (Angiogenesis Marker) ENG Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02997)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: ENG/1327

Isotype

IgG1, kappa

Immunogen

Recombinant human Endoglin protein fragment (aa74-251) (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M02997 is reactive to ENG in Human

Applications

M02997 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

70.578kDa

Background of Endoglin/CD105

CD105/Endoglin is a Type I membrane glycoprotein located on cell surfaces and is part of the TGF-beta receptor complex. This protein has been found on endothelial cells, activated macrophages, fibroblasts, and smooth-muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. CD105 is highly expressed in endothelial cells during tumor angiogenesis and inflammation, with weak or negative expression in vascular endothelium of normal tissues. Angiogenesis is a promising prognostic marker in a variety of tumors. Endoglin is a more specific and sensitive marker for tumor angiogenesis than CD31 or CD34, as it labels only newly-formed blood vessels and may serve as a prognostic marker for Prostate Adenocarcinoma, and cancers of the lung, stomach, breast, and brain.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml for 60 minutes at RT)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For ENG (Source: Uniprot.org, NCBI)

Gene Name

ENG

Full Name

Endoglin

Weight

70.578kDa

Alternative Names

CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1 ENG END, HHT1, ORW1 endoglin endoglin|CD105 antigen

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ENG, check out the ENG Infographic

ENG infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ENG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M02997 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Overexpression of transcription factor Foxa2 and Hnf1? induced rat bone mesenchymal stem cells into hepatocytes

Ren Sx, Ren Zj, Zhao My, Wang Xb, Zuo Sg, Yu F. Acta Pharmacol Sin. 2009 May;30(5):637-45. Doi: 10.1038/Aps.2009.44. Antitumor Activity Of Endogenous Mflt4 Displayed On A T4 Phage Nanoparticle Surface.

Li J, Zeng G, Qi Y, Tang X, Zhang J, Wu Z, Liang J, Shi L, Liu H, Zhang P. Plos One. 2015 Apr 7;10(4):E0123264. Doi: 10.1371/Journal.Pone.0123264. Ecollection 2015. Xenotransplantation Of Human Adipose-Derived Stem Cells In Zebrafish Embryos.

Dai J, Li Y, Zhou H, Chen J, Chen M, Xiao Z. Int J Biol Sci. 2013 Nov 21;9(10):1089-98. Doi: 10.7150/Ijbs.7367. Ecollection 2013. Genistein Promotion Of Osteogenic Differentiation Through Bmp2/Smad5/Runx2 Signaling.

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7 Customer Q&As for Anti-Endoglin / CD105 (Angiogenesis Marker) ENG Monoclonal Antibody

Question

I have attached the WB image, lot number and protocol we used for umbilical vein using anti-Endoglin / CD105 (Angiogenesis Marker) Monoclonal antibody M02997. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-16

Question

I see that the anti-Endoglin / CD105 (Angiogenesis Marker) Monoclonal antibody M02997 works with WB, what is the protocol used to produce the result images on the product page?

S. Baker

Verified customer

Asked: 2019-07-30

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-30

Question

Do you have a BSA free version of anti-Endoglin / CD105 (Angiogenesis Marker) Monoclonal antibody M02997 available?

Verified Customer

Verified customer

Asked: 2019-07-29

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Endoglin / CD105 (Angiogenesis Marker) Monoclonal antibody M02997 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-29

Question

Would anti-Endoglin / CD105 (Angiogenesis Marker) Monoclonal antibody M02997 work on monkey WB with liver?

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

Our lab technicians have not tested anti-Endoglin / CD105 (Angiogenesis Marker) Monoclonal antibody M02997 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Endoglin / CD105 (Angiogenesis Marker) Monoclonal antibody M02997 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-26

Question

My question regarding product M02997, anti-Endoglin / CD105 (Angiogenesis Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02997 anti-Endoglin / CD105 (Angiogenesis Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-03

Question

Would M02997 anti-Endoglin / CD105 (Angiogenesis Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

F. Johnson

Verified customer

Asked: 2017-04-04

Answer

You can see on the product datasheet, M02997 anti-Endoglin / CD105 (Angiogenesis Marker) Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-04-04

Question

Will anti-Endoglin / CD105 (Angiogenesis Marker) Monoclonal antibody M02997 work for WB with umbilical vein?

R. Zhao

Verified customer

Asked: 2013-05-09

Answer

According to the expression profile of umbilical vein, ENG is highly expressed in umbilical vein. So, it is likely that anti-Endoglin / CD105 (Angiogenesis Marker) Monoclonal antibody M02997 will work for WB with umbilical vein.

Boster Scientific Support

Answered: 2013-05-09

Order DetailsPrice
M02997-100ug

100 μg

$468

Free Secondary Antibody

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M02997
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$468.00

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