Anti-Eotaxin/CCL11 Antibody Picoband™

CCL11/Eotaxin antibody

Boster Bio Anti-Eotaxin/CCL11 Antibody Picoband™ catalog # PB9065. Tested in WB applications. This antibody reacts with Mouse.

Product Info Summary

SKU: PB9065
Size: 100 μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: WB

Product Name

Anti-Eotaxin/CCL11 Antibody Picoband™

View all CCL11/Eotaxin Antibodies

SKU/Catalog Number

PB9065

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Eotaxin/CCL11 Antibody Picoband™ catalog # PB9065. Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Eotaxin/CCL11 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9065)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Eotaxin recombinant protein (Position: H24-P97). Mouse Eotaxin shares 62% and 97% amino acid (aa) sequences identity with human and rat Eotaxin, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9065 is reactive to CCL11 in Mouse

Applications

PB9065 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

11 kDa

Calculated molecular weight

10.732kDa

Background of CCL11/Eotaxin

CCL11, also known as Eotaxin, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3 (CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. CCL11 is a small cytokine belonging to the CC chemokine family. It is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The effects of CCL11 are mediated by its binding to a G-protein-linked receptor known as a chemokine receptor.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For CCL11 (Source: Uniprot.org, NCBI)

Gene Name

CCL11

Full Name

Eotaxin

Weight

10.732kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin) CCL11 SCYA11 C-C motif chemokine ligand 11 eotaxin|chemokine (C-C motif) ligand 11|eosinophil chemotactic protein|eotaxin-1|small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CCL11, check out the CCL11 Infographic

CCL11 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CCL11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Eotaxin/CCL11 Antibody Picoband™

Question

My question regarding product PB9065, anti-Eotaxin/CCL11 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-24

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9065 anti-Eotaxin/CCL11 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-24

Question

Will PB9065 anti-Eotaxin/CCL11 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-02-04

Answer

As indicated on the product datasheet, PB9065 anti-Eotaxin/CCL11 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-02-04

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Eotaxin/CCL11 antibody PB9065. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-06-25

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-06-25

Question

Is this PB9065 anti-Eotaxin/CCL11 antibody reactive to the isotypes of CCL11?

Verified Customer

Verified customer

Asked: 2018-02-06

Answer

The immunogen of PB9065 anti-Eotaxin/CCL11 antibody is E.coli-derived mouse Eotaxin recombinant protein (Position: H24-P97). Mouse Eotaxin shares 62% and 97% amino acid (aa) sequences identity with human and rat Eotaxin, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-02-06

Order DetailsPrice
PB9065

100μg

$370
PB9065-10ug

10μg sample (liquid)

$99
PB9065-Biotin

100 μg Biotin conjugated

$570
PB9065-Cy3

100 μg Cy3 conjugated

$570
PB9065-Dylight488

100 μg Dylight488 conjugated

$570
PB9065-Dylight550

100 μg Dylight550 conjugated

$570
PB9065-Dylight594

100 μg Dylight594 conjugated

$570
PB9065-FITC

100 μg FITC conjugated

$570
PB9065-HRP

100 μg HRP conjugated

$570
PB9065-APC

100 μg APC conjugated

$670
PB9065-PE

100 μg PE conjugated

$670
PB9065-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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