|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-Eotaxin Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Eotaxin(CCL11) detection. Tested with WB in Mouse.|
|Cite This Product||Anti-Eotaxin Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9065)|
|Replacement Item||This antibody may replace the following items: sc-32356|sc-32355|sc-32351|sc-66808|sc-376495|sc-166469|sc-32353 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived mouse Eotaxin recombinant protein (Position: H24-P97). Mouse Eotaxin shares 62% and 97% amino acid (aa) sequences identity with human and rat Eotaxin, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||10893 MW|
|Protein Function||In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils.|
|Tissue Specificity||Expressed constitutively in the thymus. Expression inducible in the lung (type I alveolar epithelial cells), intestine, heart, spleen, kidney.|
|Alternative Names||Eotaxin;C-C motif chemokine 11;Eosinophil chemotactic protein;Small-inducible cytokine A11;Ccl11;Scya11;|
|Research Areas|||immunology|innate immunity|chemokines|beta chemokines (cc)| kits/ lysates/ other|elisa kits|cytokines and cytokine receptors elisa kits||
Background for Eotaxin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Eotaxin Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Eotaxin Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti-Eotaxin(PB9065) at 0.5ug/ml
Lane 1: Mouse Lung Tissue Lysate at40ug
Lane 2: Mouse Intestines Tissue Lysate at 40ug
Lane 3: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 11KD
Observed bind size: 11KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,