Anti-EPCR/CD201/PROCR Picoband™ Antibody


SKU PB9880
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications ELISA, WB

Overview

Product Name Anti-EPCR/CD201/PROCR Picoband™ Antibody
SKU/Catalog Number PB9880
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Endothelial protein C receptor(PROCR) detection. Tested with WB, ELISA in Human.
Cite This Product Anti-EPCR/CD201/PROCR Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9880)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Form Lyophilized
Immunogen E. coli-derived human EPCR/CD201 recombinant protein (Position: S18-S210). Human EPCR/CD201 shares 65.5% and 65.6% amino acid (aa) sequence identity with mouse and rat EPCR/CD201, respectively.
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

Western blot analysis of EPCR/CD201 expression in A549 whole cell lysates (lane 1). EPCR/CD201 at 46KD, 52KD was detected using rabbit anti- EPCR/CD201 Antigen Affinity purified polyclonal antibody (Catalog # PB9880) at0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q9UNN8
Gene Name PROCR
Protein Name Endothelial protein C receptor
Tissue Specificity Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.
Alternative Names Endothelial protein C receptor;Activated protein C receptor;APC receptor;Endothelial cell protein C receptor;CD201;PROCR;EPCR;
Subcellular Localization Membrane; Single-pass type I membrane protein.
Molecular Weight 26671 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.
Research Areas Human

*You can search these to find other products in these research areas.
Background Endothelial protein C receptor (EPCR), also known as activated protein C receptor (APC receptor), is a protein that in humans is encoded by the PROCR gene. It has also recently been designated CD201 (cluster of differentiation 201). The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. And it is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy. The encoded protein may also play a role in malarial infection and has been associated with cancer.

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Polyclonal antibody for EPCR/PROCR detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. EPCR/PROCR information: Molecular Weight: 26671 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein; Tissue Specificity: Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.
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In stock
Order Product
PB9880
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
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