Product Info Summary
SKU: | A00690-1 |
---|---|
Size: | 100μl |
Reactive Species: | Human |
Host: | Mouse |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-EphB4 Monoclonal Antibody
SKU/Catalog Number
A00690-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-EphB4 Monoclonal Antibody catalog # A00690-1. Tested in IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EphB4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00690-1)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clone Number
5B5
Immunogen
Purified recombinant extracellular fragment of human EphB4 fused with hIgGFc tag expressed in HEK293 cell line.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00690-1 is reactive to EPHB4 in Human
Applications
A00690-1 is guaranteed for IF, IHC, WB Boster Guarantee
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IF, 1:200-1:1000
Validation Images & Assay Conditions

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Western Blot (WB) analysis using EphB4 Monoclonal antibody against extracellular domain of human EphB4 (aa16-539).

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Confocal Immunofluorescence (IF) analysis of methanol-fixed HEK293 cells trasfected with EphB4-hIgGFc using EphB4 Monoclonal antibody (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
Protein Target Info & Infographic
Gene/Protein Information For EPHB4 (Source: Uniprot.Org, NCBI)
Uniprot ID
P54760
Gene ID
2050
Gene Name
EPHB4
Full Name
Ephrin type-B receptor 4
Weight
108.27kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.10; EC 2.7.10.1; EPH receptor B4; EphB4; ephrin type-B receptor 4; hepatoma transmembrane kinase; Htk; HTKephrin receptor EphB4; Mdk2; Myk1; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; Tyro11; Tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11 EPHB4 CMAVM2, HFASD, HTK, LMPHM7, MYK1, TYRO11 EPH receptor B4 ephrin type-B receptor 4|ephrin receptor EphB4|hepatoma transmembrane kinase|soluble EPHB4 variant 1|soluble EPHB4 variant 2|soluble EPHB4 variant 3|tyrosine-protein kinase TYRO11|tyrosine-protein kinase receptor HTK
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on EPHB4, check out the EPHB4 Infographic

We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for EPHB4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-EphB4 Monoclonal Antibody (A00690-1)
No publications found for A00690-1
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-EphB4 Monoclonal Antibody
Question
We are currently using anti-EphB4 Monoclonal antibody A00690-1 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
The anti-EphB4 Monoclonal antibody (A00690-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-30