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SKU:MA1039
Clonality:Monoclonal
Clone Number:GP1.4
Application:IHC-P, IHC-F
Price: $200.00
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Overview

Product Name Anti-Episialin, EMA Antibody (Monoclonal, GP1.4)
SKU/Catalog Number MA1039
Description Mouse IgG monoclonal antibody for Episialin, EMA, mucin 1, cell surface associated (MUC1) detection. Tested with IHC-P, IHC-F in Human. No cross reactivity with other proteins.
Cite This Product Anti-Episialin, EMA Antibody (Monoclonal, GP1.4) GP1.4 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1039)
Replacement Item This antibody may replace the following items: sc-10899|sc-10897|sc-20796|sc-55531|sc-48362|sc-1066|sc-271566|sc-1665|sc-271176|sc-166774|sc-65594|sc-1063|sc-56334|sc-71810|sc-71808|sc-71809|sc-71807|sc-136010|sc-130333|sc-1063-G|sc-1066-G|sc-1076-G|sc-101809|sc-101810|sc-10897|sc-10899|sc-12948-R|sc-12949|sc-12949-R|sc-130333|sc-136010|sc-20796|sc-48362|sc-55531 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human
Application IHC-P, IHC-F

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Human milk fat globule membranes.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number GP1.4
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name MUC1
Protein Name Mucin-1
Molecular Weight 122102 MW
Protein Function The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
Tissue Specificity Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform 7 is expressed in tumor cells only. .
Sequence Similarities Contains 1 SEA domain.
Subcellular Localization Apical cell membrane ; Single- pass type I membrane protein . Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.
Uniprot ID P15941
Alternative Names Mucin-1;MUC-1;Breast carcinoma-associated antigen DF3;Cancer antigen 15-3;CA 15-3;Carcinoma-associated mucin;Episialin;H23AG;Krebs von den Lungen-6;KL-6;PEMT;Peanut-reactive urinary mucin;PUM;Polymorphic epithelial mucin;PEM;Tumor-associated epithelial membrane antigen;EMA;Tumor-associated mucin;CD227;Mucin-1 subunit alpha;MUC1-NT;MUC1-alpha;Mucin-1 subunit beta;MUC1-beta;MUC1-CT;MUC1;PUM;
Research Areas tags & cell markers|cell type markers|tumor associated|signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|muc|cancer|invasion/microenvironment|tumor immunology|tumor-associated antigens|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Mucin-1

MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1(EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type I transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.

Anti-Episialin, EMA Antibody (Monoclonal, GP1.4) Images

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Anti-Episialin, EMA Antibody (Monoclonal, GP1.4)
Anti- Episialin, EMA antibody, MA1039, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti-Episialin, EMA Antibody (Monoclonal, GP1.4)
Anti- Episialin, EMA antibody, MA1039, IHC(P)
IHC(P): Human Lung Cancer Tissue
Anti-Episialin, EMA Antibody (Monoclonal, GP1.4)
Anti- Episialin, EMA antibody, MA1039, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to muc1 antibody, ema antibody, kl-6 antibody, muc-1 antibody, mucin 1 antibody, epithelial membrane antigen antibody