|Validated Species:||Human, Mouse|
Data & Images
|Product Name||Anti-EPO Receptor Antibody|
|Description||Rabbit IgG polyclonal antibody for Erythropoietin receptor(EPOR) detection. Tested with WB in Human;Mouse.|
|Cite This Product||Anti-EPO Receptor Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2032)|
|Replacement Item||This antibody may replace the following items: sc-82593|sc-697|sc-5624|sc-82590|sc-365662|sc-695 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human EPO Receptor(362-379aa EHAQDTYLVLDKWLLPRN), identical to the related mouse sequence, and different from the related rat sequence by three amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Erythropoietin receptor|
|Molecular Weight||55065 MW|
|Protein Function||Receptor for erythropoietin. Mediates erythropoietin- induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.|
|Tissue Specificity||Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early- stage erythroid progenitor cells.|
|Sequence Similarities||Belongs to the type I cytokine receptor family. Type 1 subfamily.|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein.|
|Alternative Names||Erythropoietin receptor;EPO-R;EPOR;|
Background for Erythropoietin receptor
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-EPO Receptor Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-EPO Receptor Antibody Images
Click the images to enlarge.
All lanes: Anti EPO Receptor (PA2032) at 0.5ug/ml
WB: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 55KD
Observed bind size: 55KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,