Anti-ER alpha ESR1 Rabbit Monoclonal Antibody

ER alpha/NR3A1 antibody

Boster Bio Anti-ER alpha ESR1 Rabbit Monoclonal Antibody catalog # M00057-2. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: M00057-2
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-ER alpha ESR1 Rabbit Monoclonal Antibody

View all ER alpha/NR3A1 Antibodies

SKU/Catalog Number

M00057-2

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ER alpha ESR1 Rabbit Monoclonal Antibody catalog # M00057-2. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ER alpha ESR1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00057-2)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

IE-5

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ER alpha

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00057-2 is reactive to ESR1 in Human, Mouse, Rat

Applications

M00057-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

82 kDa

Calculated molecular weight

66.216kDa

Background of ER alpha/NR3A1

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:100
ICC/IF 1:50-1:100
FC 1:30

Validation Images & Assay Conditions

Gene/Protein Information For ESR1 (Source: Uniprot.org, NCBI)

Gene Name

ESR1

Full Name

Estrogen receptor

Weight

66.216kDa

Superfamily

nuclear hormone receptor family

Alternative Names

ER alpha; ER; Era; ER-alpha; ESR1; ESRESRA; Estradiol receptor; estrogen receptor 1; estrogen receptor alpha delta 3*4,56,7*/819-2 isoform; estrogen receptor alpha delta 4 +49 isoform; estrogen receptor alpha delta 4*5,6,7*/654 isoform; estrogen receptor alpha; estrogen receptor; NR3A1; NR3A1DKFZp686N23123; Nuclear receptor subfamily 3 group A member 1 ESR1 ER, ESR, ESRA, ESTRR, Era, NR3A1 estrogen receptor 1 estrogen receptor|E2 receptor alpha|ER-alpha|estradiol receptor|estrogen nuclear receptor alpha|estrogen receptor alpha E1-E2-1-2|estrogen receptor alpha E1-N2-E2-1-2|nuclear receptor subfamily 3 group A member 1|oestrogen receptor alpha

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ESR1, check out the ESR1 Infographic

ESR1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ESR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00057-2 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effects of estradiol on liver estrogen receptor-? and its mRNA expression in hepatic fibrosis in rats

Fas counterattack in cholangiocarcinoma: A mechanism for immune evasion in human hilar cholangiocarcinomas

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2 Customer Q&As for Anti-ER alpha ESR1 Rabbit Monoclonal Antibody

Question

What is a secondary antibody for the M00057-2 antibody that is HRP or biotin conjugate?

Verified customer

Asked: 2019-11-04

Answer

For the Anti-ER alpha Rabbit Monoclonal Antibody M00057-2, we suggest Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate BA1054 and Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Biotin Conjugate BA1003 as secondary antibodies that is HRP (Horseradish Peroxidase) or biotin conjugate.

Boster Scientific Support

Answered: 2019-11-04

Question

For M00057-2 (Abways CY5041) , what is the immunogen sequence?

Verified Customer

Verified customer

Asked: 2017-11-13

Answer

The immunogen sequence for M00057-2 (Abways CY5041) is CPPLNSVSPSPLMLLHPPPQ.

Boster Scientific Support

Answered: 2017-11-13

Order DetailsPrice
M00057-2

100μL

$370
M00057-2-30ul

30μL sample

$99
M00057-2-carrier-free

Carrier Free

$370

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M00057-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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