Product Info Summary
SKU: | P00030 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-ERK/MAPK (Thr202, Tyr204) Antibody
SKU/Catalog Number
P00030
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-ERK/MAPK (Thr202, Tyr204) Antibody (Catalog # P00030). Tested in WB, IHC, ICC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-ERK/MAPK (Thr202, Tyr204) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00030)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Clone Number
JE44-80
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 of rat ERK/MAPK conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P00030 is reactive to Mapk1 in Human, Mouse, Rat
Applications
P00030 is guaranteed for IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
41.276kDa
Background of ERK2
Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC: 1:100-1:500
ICC: 1:100-1:250
Validation Images & Assay Conditions
Click image to see more details
Immunostaining of granule cells in the dentate gyrus of saline treated mouse showing ERK/MAPK when phosphorylated at Thr202/Tyr204 (red) and nuclei (blue).
Click image to see more details
Western blot of human T47D cell lysate showing specific immunolabeling of ~42-44 kDa ERK/MAPK protein phosphorylated at Thr202/Tyr204 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 min).
Protein Target Info & Infographic
Gene/Protein Information For Mapk1 (Source: Uniprot.org, NCBI)
Gene Name
Mapk1
Full Name
Mitogen-activated protein kinase 1
Weight
41.276kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.24; ERK; ERK2; ERK-2; ERK2MAP kinase isoform p42; ERT1; Extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAPK 1; MAPK1; MAPK2; mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; p38; p40; p41; p41mapk; p42mapk; p42-MAPK; PRKM1; PRKM1MAPK 2; PRKM2; protein tyrosine kinase ERK2 Mapk1|ERK-2, ERT1, Erk2, p42-MAPK|mitogen activated protein kinase 1|mitogen-activated protein kinase 1|MAP kinase 1|MAP kinase 2|MAPK 1|MAPK 2|extracellular-signal-regulated kinase 2|mitogen-activated protein kinase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Mapk1, check out the Mapk1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Mapk1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-ERK/MAPK (Thr202, Tyr204) Antibody (P00030)
Hello CJ!
P00030 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Iridoid glycosides from Morinda officinalis How. exert anti-inflammatory and anti-arthritic effects through inactivating MAPK and NF-κB signaling pathways
Panax Notoginseng Saponins Promote Osteogenic Differentiation of Bone Marrow Stromal Cells Through the ERK and P38 MAPK Signaling Pathways
Avβ3 single‐stranded DNA aptamer attenuates vascular restenosis via Ras‐PI3K/MAPK pathway in rats after percutaneous transluminal coronary angioplasty
Activation of RAW264.7 macrophages by an acidic polysaccharide derived from Citrus grandis ‘Tomentosa’
Ji F,Wang Y,Yuan J,Wu Q,Wang J,Liu D.The potential role of stromal cell-derived factor-1α/CXCR4/CXCR7 axis in adipose-derived mesenchymal stem cells.J Cell Physiol.2020 Apr;235(4):3548-3557.doi:10.1002/jcp.29243.Epub 2019 Sep 30.PMID:31566725.
Species: Rat
P00030 usage in article: APP:IF, SAMPLE: ADSCS, DILUTION:1:400
Disulfiram inhibits TGF-?-induced epithelial-mesenchymal transition and stem-like features in breast cancer via ERK/NF-?B/Snail pathway
eIF3i activity is critical for endothelial cells in tumor induced angiogenesis through regulating VEGFR and ERK translation
Sang J, Chen Y, Tao Y. Mol Med Rep. 2011 Nov-Dec;4(6):1163-7. Doi: 10.3892/Mmr.2011.535. Epub 2011 Jul 15. Nitric Oxide Inhibits Gastric Cancer Cell Growth Through The Modulation Of The Akt Pathway.
Li Xl, Chen Xq, Zhang Mn, Chen N, Nie L, Xu M, Gong J, Shen Pf, Su Zz, Weng X, Tan Jy, Zhao T, Zeng H, Zhou Q. Int J Clin Exp Pathol. 2015 Apr 1;8(4):3871-81. Ecollection 2015. Sox9 Was Involved In Tkis Resistance In Renal Cell Carcinoma Via Raf/M...
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