Anti-Esrp-2 Monoclonal Antibody

Esrp2 antibody

Boster Bio Anti-Esrp-2 Monoclonal Antibody (Catalog # M08004). Tested in ELISA, WB applications. This antibody reacts with Mouse.

Product Info Summary

SKU: M08004
Size: 100ug
Reactive Species: Mouse
Host: Mouse
Application: ELISA, WB

Customers Who Bought This Also Bought

Product Name

Anti-Esrp-2 Monoclonal Antibody

View all Esrp2 Antibodies

SKU/Catalog Number

M08004

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Esrp-2 Monoclonal Antibody (Catalog # M08004). Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Esrp-2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08004)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 22C5.H7.A6 IgG2a kappa

Isotype

IgG2a kappa

Immunogen

Anti-Esrp-2 is produced by repeated immunizations of full length recombinant mouse Esrp-2 fusion protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M08004 is reactive to Esrp2 in Mouse

Applications

M08004 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

77.361kDa

Background of Esrp2

Epithelial splicing regulatory protein 2 (Esrp-2) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-2 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:100,000
WB: 1:1000

Validation Images & Assay Conditions

Gene/Protein Information For Esrp2 (Source: Uniprot.org, NCBI)

Gene Name

Esrp2

Full Name

Epithelial splicing regulatory protein 2

Weight

77.361kDa

Superfamily

ESRP family

Alternative Names

epithelial splicing regulatory protein 2; FLJ21918; FLJ22248; RBM35BRNA-binding protein 35B; RNA binding motif protein 35A; RNA binding motif protein 35B; RNA-binding motif protein 35B Esrp2|9530027K23Rik, Rbm35, Rbm35b|epithelial splicing regulatory protein 2|epithelial splicing regulatory protein 2|RNA binding motif protein 35b|RNA-binding motif protein 35B|RNA-binding protein 35B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Esrp2, check out the Esrp2 Infographic

Esrp2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Esrp2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for M08004

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Esrp-2 Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Esrp-2 Monoclonal Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

7 Customer Q&As for Anti-Esrp-2 Monoclonal Antibody

Question

We are currently using anti-Esrp-2 Monoclonal antibody M08004 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-13

Answer

The anti-Esrp-2 Monoclonal antibody (M08004) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-13

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Esrp-2 Monoclonal antibody M08004. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-02-11

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-11

Question

Is a blocking peptide available for product anti-Esrp-2 Monoclonal antibody (M08004)?

Verified Customer

Verified customer

Asked: 2019-09-13

Answer

We do provide the blocking peptide for product anti-Esrp-2 Monoclonal antibody (M08004). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-13

Question

I was wanting to use your anti-Esrp-2 Monoclonal antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?

Verified Customer

Verified customer

Asked: 2018-09-18

Answer

It shows on the product datasheet, M08004 anti-Esrp-2 Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-09-18

Question

Would M08004 anti-Esrp-2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

M. Anderson

Verified customer

Asked: 2018-01-09

Answer

It shows on the product datasheet, M08004 anti-Esrp-2 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-01-09

Question

See attached the WB image, lot number and protocol we used for liver using anti-Esrp-2 Monoclonal antibody M08004. Please let me know if you require anything else.

C. Parker

Verified customer

Asked: 2016-01-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-01-11

Question

My question regarding product M08004, anti-Esrp-2 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

A. Thomas

Verified customer

Asked: 2014-06-18

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M08004 anti-Esrp-2 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-06-18

Order DetailsPrice
M08004-100uL

100uL

$612

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
M08004
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$612.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.